Jimoh O A
Animal Physiology Laboratory, Department of Animal Science, University of Ibadan, Ibadan, Nigeria.
Agricultural Technology Department, Federal Polytechnic Ado Ekiti, Ado Ekiti, Ekiti State, Nigeria.
Trop Anim Health Prod. 2020 Jan;52(1):249-255. doi: 10.1007/s11250-019-02011-z. Epub 2019 Jul 13.
Coconut water as a natural diluent was evaluated on rabbit semen quality, conception and litter size in artificially inseminated does. A total of 10 bucks and 90 does, all crossbred New Zealand White × chinchilla, were used for the trial. Pooled semen was obtained from bucks using artificial vagina. Semen diluents were prepared at 0%, 20%, 40%, 60% and 80% and control 50% normal saline and designated as T1, T2, T3, T4, T5 and T6 respectively, in a completely randomised design. Semen analysis was conducted on fresh semen and the various diluted semen immediately at 37 °C. Fifteen does each were randomly allotted to the different treatment and were inseminated with their respective treatment diluted semen using the standard procedure, and conception rate, litter size and productivity index at birth were assessed at kindling. Result obtained revealed that spermatozoa motility and structural membrane integrity of the diluted semen were significantly (p < 0.05) affected by the percentage of dilution. The range of values obtained for spermatozoa motility, structural membrane integrity and acrosome integrity is within the accepted values for good-quality semen. Conception rate, litter size and productivity index at birth were highest in does inseminate with T4 (60%), and the least values were obtained in T5 (80%). In conclusion, coconut water is a suitable diluent for rabbit semen for on-farm artificial insemination, the optimal dilution rate of coconut water in rabbit semen is 60%, which guarantees high conception, litter size and productivity index at birth.
在人工授精的母兔中,评估了椰子水作为天然稀释剂对兔精液质量、受孕率和产仔数的影响。总共使用了10只公兔和90只母兔,均为新西兰白兔×龙猫的杂交品种进行试验。使用人工阴道从公兔采集混合精液。以0%、20%、40%、60%和80%的比例制备精液稀释剂,并设50%生理盐水作为对照,分别指定为T1、T2、T3、T4、T5和T6,采用完全随机设计。在37℃下立即对新鲜精液和各种稀释精液进行精液分析。将15只母兔随机分配到不同处理组,采用标准程序用各自处理的稀释精液进行人工授精,并在分娩时评估受孕率、产仔数和出生时的生产力指数。结果表明,稀释精液的精子活力和结构膜完整性受稀释百分比的显著影响(p < 0.05)。精子活力、结构膜完整性和顶体完整性的取值范围在优质精液的可接受范围内。用T4(60%)稀释精液进行人工授精的母兔受孕率、产仔数和出生时的生产力指数最高,而T5(80%)组的值最低。总之,椰子水是适合农场兔精液人工授精的稀释剂,椰子水在兔精液中的最佳稀释率为60%,可确保高受孕率、产仔数和出生时的生产力指数。