Cross N L
Department of Physiological Sciences, Oklahoma State University, Stillwater 74074, USA.
Biol Reprod. 1996 Jan;54(1):138-45. doi: 10.1095/biolreprod54.1.138.
Seminal plasma inhibits human sperm from developing the ability to undergo the acrosome reaction. The inhibitory activity was identified as that of cholesterol on the basis of its solubility in organic solvents, its chromatographic behavior (adsorption, thin-layer, and gas chromatography), and its mass spectrum. Contrary to findings in other reports, no evidence for inhibitory proteins or peptides was found, and spermine was not an effective inhibitor. The inhibitory activity of untreated seminal plasma from individual ejaculates was highly correlated with the cholesterol content of the ejaculates (r = 0.96), suggesting that the amount of cholesterol determines the inhibitory activity of unfractionated seminal plasma. The inhibitory activity of unfractionated seminal plasma was significantly less, relative to the cholesterol content, than the activity of pure cholesterol, which is consistent with the idea that there are components in seminal plasma that partially counter the effect of cholesterol by promoting the development of acrosomal responsiveness.
精浆会抑制人类精子产生顶体反应的能力。基于胆固醇在有机溶剂中的溶解性、其色谱行为（吸附、薄层和气相色谱）以及其质谱，确定这种抑制活性就是胆固醇的活性。与其他报告中的发现相反，未发现抑制性蛋白质或肽的证据，精胺也不是有效的抑制剂。来自个体射精的未处理精浆的抑制活性与射精中的胆固醇含量高度相关（r = 0.96），这表明胆固醇的量决定了未分级精浆的抑制活性。相对于胆固醇含量，未分级精浆的抑制活性明显低于纯胆固醇的活性，这与精浆中存在通过促进顶体反应性发展来部分抵消胆固醇作用的成分的观点一致。
