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柑橘溃疡病菌(哈斯)道森培养过程中游离氨基酸库的组成。

The free amino acid pool composition during growth of the culture of Xanthomonas citri (Hasse) Dowson.

作者信息

Prasad M

出版信息

Zentralbl Bakteriol Naturwiss. 1979;134(8):692-6. doi: 10.1016/s0323-6056(79)80029-4.

DOI:10.1016/s0323-6056(79)80029-4
PMID:120748
Abstract

Fourteen amino acids were found to constitute the intracellular amino compound pool of the developing culture of Xanthomonas citri (Hasse) Dowson. These were serine, dl-alanine, beta-alanine, leucine/isoleucine, threonine, aspartic acid, glutamic acid, asparagine, glutamine, cystine, histidine, tyrosine, tryptophan, and proline. Of these, beta-alanine, threonine, aspartic acid, glutamic acid, asparagine, and tyrosine could be traced as excretory amino acids in the bacterial culture filtrate, being exuded at the cost of and parallel to the depletion of their amounts in bacterial cell extract. Thus, the composition and pool size of intra- and extracellular amino acids varies considerably during the growth of this pathogen. The wall constituents of X. citri, especially alanine, glutamic acid, and aspartic acid, were prominent among the amino compounds detected.

摘要

已发现14种氨基酸构成了柑橘黄龙病菌(哈斯)道森发育中的培养物的细胞内氨基化合物库。这些氨基酸分别是丝氨酸、dl-丙氨酸、β-丙氨酸、亮氨酸/异亮氨酸、苏氨酸、天冬氨酸、谷氨酸、天冬酰胺、谷氨酰胺、胱氨酸、组氨酸、酪氨酸、色氨酸和脯氨酸。其中,β-丙氨酸、苏氨酸、天冬氨酸、谷氨酸、天冬酰胺和酪氨酸可在细菌培养滤液中作为排泄性氨基酸被追踪到,它们以细菌细胞提取物中其含量的消耗为代价并与之平行地渗出。因此,在这种病原菌的生长过程中,细胞内和细胞外氨基酸的组成和库大小有很大变化。在检测到的氨基化合物中,柑橘黄龙病菌的细胞壁成分,尤其是丙氨酸、谷氨酸和天冬氨酸很突出。

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