Chen Long, Ren Wen-Hua, Zhu Shan-Liang, Gao Wei, Zhou Juan, Jiang Ying-Zi, Gu Yu
College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Sheng Li Xue Bao. 2002 Jun 25;54(3):258-62.
Sixty healthy Sprague-Dawley male rats were used and divided randomly into control group (group C), cadmium loading group with medium dose (group M) and cadmium loading group with high dose (group H). Groups C, M and H were orally dosed daily with 0, 5 and 10 mg/kg of cadmium for over 6 weeks. Effects of cadmium loading on testis and endocrine function of reproduction in male rats were studied. The results showed that the zinc content decreased slightly in testis and plasma, and the cadmium concentration increased significantly in the testis of groups M and H; while the plasma levels of cadmium and zinc had no obvious difference as compared with those of group C; daily sperm production in the testis of group H decreased markedly during week 3 of cadmium loading, and was significantly lower in groups M and H as compared to that in group C during week 6; alkaline phosphatase (ALP) in group H and lactate dehydrogenase-X (LDH-X) in groups M and H were markedly lower compared to those of group C; plasma testosterone (T) level in both cadmium loading groups decreased and was low or significantly lower than that in group C; follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels had no apparent difference between the three groups. It is suggested that the gradual accumulation of cadmium in testis tissue induced by chronic cadmium loading results in changes in some enzyme activity, a decrease in sperm production, and defect of endocrine function activity in the testis.
选用60只健康的Sprague-Dawley雄性大鼠，随机分为对照组（C组）、中剂量镉染毒组（M组）和高剂量镉染毒组（H组）。C组、M组和H组大鼠每天分别经口灌胃给予0、5和10 mg/kg镉，持续6周以上。研究镉染毒对雄性大鼠睾丸及生殖内分泌功能的影响。结果显示，M组和H组大鼠睾丸及血浆中锌含量略有下降，睾丸中镉浓度显著升高；而血浆镉和锌水平与C组相比无明显差异；镉染毒第3周时，H组大鼠睾丸每日精子生成量明显下降，染毒第6周时，M组和H组大鼠睾丸每日精子生成量显著低于C组；H组碱性磷酸酶（ALP）以及M组和H组乳酸脱氢酶-X（LDH-X）均明显低于C组；两个镉染毒组大鼠血浆睾酮（T）水平均下降，且低于或显著低于C组；三组大鼠促卵泡生成素（FSH）和促黄体生成素（LH）水平无明显差异。提示慢性镉染毒导致镉在睾丸组织中逐渐蓄积，引起睾丸某些酶活性改变、精子生成减少以及睾丸内分泌功能活动缺陷。
