Bawden M P, Palmer T T, Leef M F, Beaudoin R L
Bull World Health Organ. 1979;57 Suppl 1(Suppl):205-9.
Vaccination against Plasmodium falciparum with attenuated sporozoites is the goal of the US Navy's Malaria Vaccine Program. One requirement in the development of this vaccine is an immunological test to study the sporozoite antigen and immune responses it induces. Using an indirect fluorescent antibody test (IFAT) and P. berghei in the mouse or rat as a model, we have made significant progress toward this goal. Four antigens were detected in vaccine preparations: sporozoite-specific antigens, mosquito antigens, antigens on the sporozoite that are common to erythrocytic stages, and bovine serum albumm, an antigenic element of the isolation medium no longer employed. The IFAT was a reliable monitor of vaccination in a mouse and rat model in conjunction with protection to challenge. The test was a sensitive monitor of vaccine quality. Anamnestic responses to bites of infected mosquitos were detected in mice previously immunized with irradiated sporozoites.
用减毒疟原虫子孢子进行疟疾疫苗接种是美国海军疟疾疫苗计划的目标。开发这种疫苗的一个要求是进行一项免疫学检测，以研究子孢子抗原及其诱导的免疫反应。我们以小鼠或大鼠为模型，使用间接荧光抗体试验（IFAT）和伯氏疟原虫，在这一目标上取得了重大进展。在疫苗制剂中检测到四种抗原：子孢子特异性抗原、蚊子抗原、红细胞期共有的子孢子抗原，以及牛血清白蛋白（一种不再使用的分离培养基的抗原成分）。在小鼠和大鼠模型中，IFAT是接种疫苗及挑战保护的可靠监测指标。该检测是疫苗质量的敏感监测指标。在用辐照子孢子预先免疫的小鼠中，检测到对感染蚊子叮咬的回忆反应。
