通过间接免疫荧光法对疟原虫表面抗原进行表征：检测阶段特异性和种特异性抗疟抗体。
Characterization of sporozoite surface antigens by indirect immunofluorescence: detection of stage- and species-specific antimalarial antibodies.
作者信息
Nardin E, Gwadz R W, Nussenzweig R S
出版信息
Bull World Health Organ. 1979;57 Suppl 1(Suppl):211-7.
Abstract
Indirect immunofluorescence (IF) was used to localize stage-specific antigen(s) on the surface of the sporozoite membrane. The authors examined the feasibility of using an IF assay to determine whether an antisporozoite response is developed by individuals living in endemic areas. The specificity and sensitivity of the IF assay were first defined by using hyperimmune sera of sporozoite-immunized hosts protected against rodent (P. berghei), simian (P. knowlesi), and human (P. falciparum, P. vivax) malarias. Species- and stage-specific antisporozoite antibodies were detected by this technique when viable or glutaralde-hyde-fixed sporozoites were used as antigen. The sensitivity of indirect immunofluorescence was found to be 5-10 times greater than that of the circumsporozoite precipitation (CSP) assay. Preliminary studies on sera obtained from individuals living in endemic areas showed that the IF assay can be used to detect species- and stage-specific antisporozoite antibodies in sera of naturally-infected human and simian hosts.
摘要
间接免疫荧光法（IF）用于定位子孢子膜表面的阶段特异性抗原。作者研究了使用IF检测法来确定生活在流行地区的个体是否产生抗子孢子反应的可行性。首先通过使用针对啮齿动物（伯氏疟原虫）、灵长类动物（诺氏疟原虫）和人类（恶性疟原虫、间日疟原虫）疟疾具有免疫力的子孢子免疫宿主的超免疫血清来确定IF检测法的特异性和敏感性。当使用活的或经戊二醛固定的子孢子作为抗原时，通过该技术可检测到种属和阶段特异性的抗子孢子抗体。发现间接免疫荧光法的敏感性比环子孢子沉淀（CSP）检测法高5至10倍。对来自流行地区个体的血清进行的初步研究表明，IF检测法可用于检测自然感染的人类和灵长类动物宿主血清中的种属和阶段特异性抗子孢子抗体。
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