[Establishment of Hymenolepis diminuta-animal model and morphology of cysticercoid].

AIM

To observe the morphological changes in the process of the development of Hymenolepis diminuta.

METHODS

The life cycle of Hymenolepis diminuta was established between Rattus domesticus albus and Triboliun castaneum. The morphology of cysticercoid were observed microscopically, and the ultrastructure of the body surface of cysticercoid were observed by scanning electron microscopy.

RESULTS

Three phases including the mature stage, the blister stage and the protective outer membrane-forming stage during the growing course of cysticercoid were observed microscopically. Under scanning electron microscope, lots of sieve-like micropores on the surface of mature cysticercoid were seen in the second week after infection. The blister phase was found in the third week and the outer membrane measuring about 45 microns in thickness were found surrounding the cysticercoid and vesicular surface, forming a smooth cyst wall in the fourth week.

CONCLUSION

The life cycle of Hymenolepis diminuta has been established in the animal-model. The finding of the three phases during the growing course of cysticercoid is reported for the first time.