Wang Q, Wang M, Chang H, Yang B
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1998;16(3):189-92.
To explore whether the difference in the amount of chloroquine (CQ) accumulated in CQ-resistant (CR) and in CQ-sensitive (CS) P. berghei is involved in the infected erythrocytes (RBC) or in the parasites.
The concentration of CQ in infected RBC and parasites following ig administration of CQ to CS and CR infected mice was determined by HPLC method (extra standard).
3 h after 4.06 mg/kg and 400 mg/kg administration, the accumulation of CQ in both infected RBC was similar, but in CR was 54.0% and 42.1% less than in CS (P < 0.001) respectively. The CQ accumulation in CS 3 h and 7 h after ig administration of CQ 400 mg/kg was similar (0.780 +/- 0.128 nmol/protein and 0.760 +/- 0.180 nmol/mg protein), while in CR the CQ-accumulation was higher at 7 h than that at 3 h after CQ administration (0.452 +/- 0.079 nmol/mg protein and 0.559 +/- 0.124 nmol/mg protein P < 0.02).
The low CQ accumulation in CR is attributable to the parasite rather than to the infected RBC, and CR was not found to excrete CQ more rapidly than CS.
探讨氯喹（CQ）抗性（CR）和敏感性（CS）伯氏疟原虫中积累的氯喹量的差异是与被感染红细胞（RBC）还是与疟原虫有关。
通过高效液相色谱法（外标法）测定对CS和CR感染小鼠经口给予CQ后，被感染红细胞和疟原虫中CQ的浓度。
给予4.06mg/kg和400mg/kg后3小时，两种感染红细胞中CQ的积累相似，但CR中的积累分别比CS中的少54.0%和42.1%（P<0.001）。给予400mg/kg CQ经口给药后3小时和7小时，CS中CQ的积累相似（0.780±0.128nmol/蛋白和0.760±0.180nmol/mg蛋白），而在CR中，给药后7小时的CQ积累高于3小时（0.452±0.079nmol/mg蛋白和0.559±0.124nmol/mg蛋白，P<0.02）。
CR中CQ积累较低归因于疟原虫而非被感染的红细胞，且未发现CR比CS更快地排泄CQ。
