Wang Q, Wang M, Chang H, Yang B
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine Shanghai, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):228-30.
To isolate and quantitate chloroquine (CQ)-binding proteins in both CQ-sensitive (CS) AND CQ-resistant (CR) Plasmodium berghei.
P. berghei infected mice were each given i.g. CQ 400 mg/kg, respectively, 3 h later, the proteins of the parasites collected were separated using Ultrogel AcA34 gel column, followed by CQ extraction, the CQ concentration was determined by HPLC (extra standard).
There were 58 protein peaks in the CS, all of which were bound with CQ, fraction Nos. 64-86 (peak 9) being the predominant, accounting for 16.4% of the total amount of CQ-binding protein. In the CR there were 40 protein peaks, among which 32 were bound with CQ, the predominant fraction Nos. 60-83 (peaks 6, 7), had a higher CQ-binding ability than CS.
The distribution and amount of each CQ-binding protein peak in both CS and CR strains of P. berghei were demonstrated, exhibiting differences in the ability of CQ-binding not only between CS and CR strains but also within the same strain.
分离并定量测定氯喹敏感（CS）和氯喹耐药（CR）伯氏疟原虫中的氯喹（CQ）结合蛋白。
给感染伯氏疟原虫的小鼠分别经口给予400mg/kg氯喹，3小时后，收集的寄生虫蛋白用Ultrogel AcA34凝胶柱分离，随后进行氯喹提取，用高效液相色谱法（外标法）测定氯喹浓度。
CS中有58个蛋白峰，均与氯喹结合，第64 - 86号组分（峰9）为主峰，占氯喹结合蛋白总量的16.4%。CR中有40个蛋白峰，其中32个与氯喹结合，第60 - 83号主要组分（峰6、峰7）的氯喹结合能力高于CS。
证实了伯氏疟原虫CS和CR株中各氯喹结合蛋白峰的分布和含量，显示出CS和CR株之间以及同一株内氯喹结合能力存在差异。
