Hussey Patrick J, Allwood Ellen G, Smertenko Andrei P
The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK.
Philos Trans R Soc Lond B Biol Sci. 2002 Jun 29;357(1422):791-8. doi: 10.1098/rstb.2002.1086.
The plant actin cytoskeleton is a highly dynamic, fibrous structure essential in many cellular processes including cell division and cytoplasmic streaming. This structure is stimulus responsive, being affected by internal stimuli, by biotic and abiotic stresses mediated in signal transduction pathways by actin-binding proteins. The completion of the Arabidopsis genome sequence has allowed a comparative identification of many actin-binding proteins. However, not all are conserved in plants, which possibly reflects the differences in the processes involved in morphogenesis between plant and other cells. Here we have searched for the Arabidopsis equivalents of 67 animal/fungal actin-binding proteins and show that 36 are not conserved in plants. One protein that is conserved across phylogeny is actin-depolymerizing factor or cofilin and we describe our work on the activity of vegetative tissue and pollen-specific isoforms of this protein in plant cells, concluding that they are functionally distinct.
植物肌动蛋白细胞骨架是一种高度动态的纤维状结构，在包括细胞分裂和细胞质流动在内的许多细胞过程中至关重要。这种结构对刺激有反应，会受到内部刺激以及由肌动蛋白结合蛋白介导的信号转导途径中的生物和非生物胁迫的影响。拟南芥基因组序列的完成使得对许多肌动蛋白结合蛋白进行比较鉴定成为可能。然而，并非所有这些蛋白在植物中都是保守的，这可能反映了植物与其他细胞在形态发生过程中所涉及的过程的差异。在这里，我们寻找了67种动物/真菌肌动蛋白结合蛋白在拟南芥中的对应物，并表明其中36种在植物中不保守。一种在整个系统发育中都保守的蛋白是肌动蛋白解聚因子或丝切蛋白，我们描述了我们对该蛋白在植物细胞中的营养组织和花粉特异性同工型活性的研究工作，得出的结论是它们在功能上是不同的。
