Vantard Marylin, Blanchoin Laurent
Laboratoire de Physiologie Cellulaire Végétale, UMR 5019, CEA/CNRS/UJF, DRDC-CEA de Grenoble, 17 rue des martyrs, 38054, Grenoble, France.
Curr Opin Plant Biol. 2002 Dec;5(6):502-6. doi: 10.1016/s1369-5266(02)00300-x.
Growing evidence shows that the actin cytoskeleton is a key effector of signal transduction, which controls and maintains the shape of plant cells, as well as playing roles in plant morphogenesis. Recently, several signaling pathways, including those triggered by hormones, Ca(2+), and cAMP, have been reported to be connected to the reorganization of the actin cytoskeleton. The molecular mechanisms involved in such signaling cascades are, however, largely unknown. The Arabidopsis genome sequence is a valuable tool for identifying some of the highly conserved molecules that are involved in such signaling cascades. Recent work has begun to unravel these complex pathways using a panoply of techniques, including genetic analysis, live-cell imaging of intracellular actin dynamics, in vivo localization of factors that are involved in the control of actin dynamics, and the biochemical characterization of how these factors function.
越来越多的证据表明，肌动蛋白细胞骨架是信号转导的关键效应器，它控制并维持植物细胞的形状，同时在植物形态发生中发挥作用。最近，据报道，包括由激素、Ca(2+)和cAMP触发的信号通路在内的几种信号通路与肌动蛋白细胞骨架的重组有关。然而，参与此类信号级联反应的分子机制在很大程度上尚不清楚。拟南芥基因组序列是鉴定参与此类信号级联反应的一些高度保守分子的宝贵工具。最近的研究工作已经开始使用一系列技术来揭示这些复杂的信号通路，这些技术包括遗传分析、细胞内肌动蛋白动力学的活细胞成像、参与肌动蛋白动力学控制的因子的体内定位，以及这些因子如何发挥作用的生化特性分析。
