一种用于普通小麦（Triticum aestivum L.）品种指纹图谱分析的简化扩增片段长度多态性（AFLP）方法。

A simplified AFLP method for fingerprinting of common wheat (Triticum aestivum L.) cultivars.

作者信息

Tyrka Mirosław

机构信息

Institute of Genetics and Plant Breeding, Agricultural University, Lublin, Poland.

出版信息

J Appl Genet. 2002;43(2):131-43.

PMID:12080170

Abstract

The simplified AFLP method was developed and evaluated for identification and genetic diversity studies of wheat cultivars. Selective primers exploited in AFLP assay based on a single cutting enzyme PstI ((PstI)AFLP) generated total of 111 robust fragments, including 67 (60%) monomorphic and 12 (11%) cultivar-specific markers. Average similarity between 15 cultivars was 0.650, and varied from 0.293 ('Hope' vs. 'Aurora') to 0.865 ('Norman' vs. 'Hornet'). Mean similarities within groups of winter wheat cultivars with and without 1BL/1RS chromosome were 0.713 and 0.685, respectively. A higher variation was found in the group of spring wheats: 0.677. The obtained results confirm the usefulness of the proposed modification of the AFLP technique for diversity studies and identification of common wheat cultivars.

摘要

开发并评估了简化的扩增片段长度多态性（AFLP）方法，用于小麦品种的鉴定和遗传多样性研究。基于单一切割酶PstI的AFLP分析中使用的选择性引物（(PstI)AFLP）共产生了111个稳定片段，其中包括67个（60%）单态性片段和12个（11%）品种特异性标记。15个品种之间的平均相似性为0.650，范围从0.293（“Hope”与“Aurora”）到0.865（“Norman”与“Hornet”）。具有和不具有1BL/1RS染色体的冬小麦品种组内的平均相似性分别为0.713和0.685。在春小麦组中发现了更高的变异性：0.677。所得结果证实了所提出的AFLP技术改进方法在普通小麦品种多样性研究和鉴定中的实用性。