Kozlova S A, Khlestkina E K, Salina E A
Genetika. 2009 Jan;45(1):92-6.
In this work, we analyzed 54 domestic cultivars of hexaploid (common) wheat Triticum aestivum L. (AABBDD genome) and accessions of tetraploid wheats of the Timopheevi group (AAGG) and rye Secale cereale (RR) using 21 SNP markers for common wheat. It was demonstrated that application of the SNP markers developed and verified for particular common wheat cultivars in allele-specific PCR analysis of other cultivars with different geographic origins could lead to an incorrect estimation of the similarity between the genotypes tested. The studied SNP markers of common wheat are inappropriate for analyzing genomes of other cereal species, in particular, Timopheevi wheats and rye S. cereale.
在本研究中,我们使用21个普通小麦的单核苷酸多态性(SNP)标记,分析了54个六倍体(普通)小麦品种(Triticum aestivum L.,基因组为AABBDD)、提莫菲维组四倍体小麦(AAGG)以及黑麦(Secale cereale,RR)。结果表明,在对具有不同地理起源的其他品种进行等位基因特异性PCR分析时,将针对特定普通小麦品种开发并验证的SNP标记用于其他品种,可能会导致对所测试基因型之间相似性的错误估计。所研究的普通小麦SNP标记不适用于分析其他谷类物种的基因组,尤其是提莫菲维小麦和黑麦。