Muravyov A V, Draygin S V, Eremin N N, Muravyov A A
Department of Medicine and Biology, Yaroslavl University, Yaroslavl, Russia.
Clin Hemorheol Microcirc. 2002;26(3):183-8.
Previous studies have shown a difference in rheological properties of young versus senescent RBCs. There are data that the athletes blood has more young RBCs than untrained people. Our research was a comparative study of the microrheological properties of young and old RBCs in athletes and in untrained people that was as control group. In athletes (men, n=24) and group of the control (men, n=20) the following parameters were measured: RBC aggregation (ARBC; Myrenne aggregometer) and deformability, RBC suspension and plasma viscosity as well as osmolarity, albumin, globulin and fibrinogen concentration, MCHC. Red cells were density (i.e., age) fractionated by the method of Murphy. After centrifugation the top 10% of the packed cell column (RBCtop, relatively young cells) and the bottom 10% (RBCbot, relatively old cells) were resuspended at 40.0+/-0.4% (in plasma) for aggregation, deformation and suspension viscosity measurements. It was found significant difference in aggregation and rigidity of the all RBC subpopulations between athletes and control group. The difference in aggregation was associated with reduced fibrinogen and increased ratio albumin/globulin in athletes. Besides, the correlation between aggregation RBCtop and RBCbot with fibrinogen was decreased in athletes. It was one of the cause of high fluidity of the RBCtop- and RBCbot suspensions and whole blood in athletes and more effective oxygen transport than in untrained people.
先前的研究表明，年轻红细胞与衰老红细胞的流变学特性存在差异。有数据显示，运动员血液中的年轻红细胞比未受过训练的人更多。我们的研究是一项比较运动员和作为对照组的未受过训练的人群中年轻和衰老红细胞微观流变学特性的研究。在运动员（男性，n = 24）和对照组（男性，n = 20）中，测量了以下参数：红细胞聚集（ARBC；Myrenne血细胞聚集仪）和变形性、红细胞悬浮液和血浆粘度以及渗透压、白蛋白、球蛋白和纤维蛋白原浓度、平均血红蛋白浓度（MCHC）。红细胞通过墨菲方法按密度（即年龄）进行分级分离。离心后，将压实细胞柱顶部的10%（RBCtop，相对年轻的细胞）和底部的10%（RBCbot，相对衰老的细胞）重悬于40.0±0.4%（在血浆中），用于聚集、变形和悬浮粘度测量。发现运动员组和对照组之间所有红细胞亚群的聚集和刚性存在显著差异。聚集差异与运动员体内纤维蛋白原减少和白蛋白/球蛋白比值增加有关。此外，运动员中RBCtop和RBCbot与纤维蛋白原之间的聚集相关性降低。这是运动员中RBCtop和RBCbot悬浮液以及全血流动性高且比未受过训练的人更有效地进行氧运输的原因之一。
