Bader D L, Ohashi T, Knight M M, Lee D A, Sato M
IRC in Biomedical Materials and Medical Engineering Division, Queen Mary, University of London, UK.
Biorheology. 2002;39(1-2):69-78.
This paper presents a series of techniques, which examine the deformation characteristics of bovine articular chondrocytes. The direct contact approach employs well established methodology, involving AFM and micropipette aspiration, to yield structural properties of local regions of isolated chondrocytes. The former technique yields a non-linear response with increased structural stiffness in a central location on a projected image of the chondrocyte. A simple viscoelastic model can be used with data from the micropipette aspiration technique to yield a mean value of Young's modulus, which is similar to that recently reported (Jones et al., 1999). An indirect approach is also described, involving the response of chondrocytes seeded within compressed agarose constructs. For 1% agarose constructs, the resulting cell strain, yields a gross cell modulus of 2.7 kPa. The study highlights the difficulties in establishing unique mechanical parameters, which reflect the deformation behaviour of articular chondrocytes.
本文介绍了一系列用于研究牛关节软骨细胞变形特征的技术。直接接触法采用了成熟的方法，包括原子力显微镜（AFM）和微吸管抽吸技术，以获取分离软骨细胞局部区域的结构特性。前一种技术在软骨细胞投影图像的中心位置产生非线性响应，且结构刚度增加。一个简单的粘弹性模型可与微吸管抽吸技术的数据结合使用，以得出杨氏模量的平均值，该值与最近报道的值（Jones等人，1999年）相似。本文还描述了一种间接方法，涉及接种在压缩琼脂糖构建体中的软骨细胞的反应。对于1%琼脂糖构建体，所产生的细胞应变得出的总细胞模量为2.7 kPa。该研究突出了确定反映关节软骨细胞变形行为的独特力学参数的困难。
