Centre for Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.
Centre for Predictive in vitro Models, Queen Mary University of London, London, UK.
Methods Mol Biol. 2023;2598:157-176. doi: 10.1007/978-1-0716-2839-3_12.
Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.
初级纤毛调节和协调多种细胞信号通路，这些通路对软骨细胞的生理学和软骨发育、健康和疾病都很重要。尽管如此，软骨细胞的初级纤毛及其在软骨生物学中的相关作用仍知之甚少。阐明软骨细胞中初级纤毛的结构和功能的关键是能够可视化这一独特结构。在这里，我们描述了用于免疫荧光标记、显微镜观察和测量软骨细胞初级纤毛的材料和方法。
