Arai Y T, Takahashi H, Kameoka Y, Shiino T, Wimalaratne O, Lodmell D L
Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.
Acta Virol. 2001;45(5-6):327-33.
Thirty-four suspected rabid brain samples from 2 humans, 24 dogs, 4 cats, 2 mongooses, I jackal and I water buffalo were collected in 1995-1996 in Sri Lanka. Total RNA was extracted directly from brain suspensions and examined using a one-step reverse transcription-polymerase chain reaction (RT-PCR) for the rabies virus nucleoprotein (N) gene. Twenty-eight samples were found positive for the virus N gene by RT-PCR and also for the virus antigens by fluorescent antibody (FA) test. Rabies virus isolates obtained from different animal species in different regions of Sri Lanka were genetically homogenous. Sequences of 203 nucleotides (nt)-long RT-PCR products obtained from 16 of 27 samples were found identical. Sequences of 1350 nt of N genes of 14 RT-PCR products were determined. The Sri Lanka isolates under study formed a specific cluster that included also an earlier isolate from India but did not include the known isolates from China, Thailand, Malaysia, Israel, Iran, Oman, Saudi Arabia, Russia, Nepal, Philippines, Japan and from several other countries. These results suggest that one type of rabies virus is circulating among human, dog, cat, mongoose, jackal and water buffalo living near Colombo City and in other five remote regions in Sri Lanka.
1995年至1996年期间，在斯里兰卡采集了34份疑似狂犬病的脑样本，这些样本来自2名人类、24只狗、4只猫、2只獴、1只胡狼和1只水牛。直接从脑悬液中提取总RNA，并使用一步法逆转录聚合酶链反应（RT-PCR）检测狂犬病病毒核蛋白（N）基因。通过RT-PCR检测发现28份样本的病毒N基因呈阳性，通过荧光抗体（FA）试验检测发现这些样本的病毒抗原也呈阳性。从斯里兰卡不同地区不同动物物种分离得到的狂犬病病毒在基因上是同源的。从27份样本中的16份获得的203个核苷酸（nt）长的RT-PCR产物序列相同。测定了14份RT-PCR产物的N基因1350 nt的序列。所研究的斯里兰卡分离株形成了一个特定的簇，该簇还包括一个早期来自印度的分离株，但不包括来自中国、泰国、马来西亚、以色列、伊朗、阿曼、沙特阿拉伯、俄罗斯、尼泊尔、菲律宾、日本以及其他几个国家的已知分离株。这些结果表明，一种类型的狂犬病病毒在科伦坡市附近以及斯里兰卡其他五个偏远地区的人类、狗、猫、獴、胡狼和水牛中传播。
