Bourlet T, Berthelot P, Grattard F, Genin C, Lucht F R, Pozzetto B
Groupe Immunité des Muqueuses et Agents Pathogènes', Faculty of Medicine Jacques Lisfranc, University of Saint-Etienne, Saint-Etienne, France.
Clin Microbiol Infect. 2002 Jun;8(6):352-7. doi: 10.1046/j.1469-0691.2002.00425.x.
To investigate the presence of the genome of GB virus C/hepatitis G virus (GBV-C/HGV) in semen and saliva from HIV-1-infected men.
Samples of blood from 33 men seropositive for HIV-1 were tested for the presence of GBV-C/HGV markers of infection, RNA by RT-PCR, and anti-E2 antibodies by ELISA, respectively. The cell-free fractions of seminal fluid and saliva samples of the patients with positive blood samples for GBV-C/HGV RNA or anti-E2 antibodies were then analyzed for the presence of the RNA of this virus. In addition, six semen samples and 11 saliva samples from GBV-C/HGV-negative men were tested.
The GBV-C/HGV RNA tested by RT-PCR was recovered from blood in 11 patients of 33 (33.3%), and the antibodies to E2 envelope protein were detected in six patients (18.2%). Since no patient was positive for both markers, the overall prevalence of GBV-C/HGV infection was 51.5% in the studied population. Four-all belonging to the homosexual risk group-of the 17 men with markers to GBV-C/HGV in blood were found to be positive for GBV-C/HGV RNA in mucosal samples: two of them exhibited genomic RNA in both semen and saliva, and two others were positive for semen only. The absence of inhibitors of the PCR technique was confirmed in all mucosal fractions found negative for GBV-C/HGV RNA, except for one saliva sample and one seminal fluid sample.
These results confirm the high prevalence of GBV-C/HGV infection in patients infected with HIV-1 by sexual exposure and the presence of GBV-C/HGV RNA in seminal fluid and saliva of men with markers of this virus in the blood, suggesting that mucosal fluids could be a potential source for the spread of the GBV-C/HGV infection.
研究人类免疫缺陷病毒1型（HIV-1）感染男性精液和唾液中GB病毒C/庚型肝炎病毒（GBV-C/HGV）基因组的存在情况。
分别对33例HIV-1血清学阳性男性的血液样本进行GBV-C/HGV感染标志物检测，采用逆转录聚合酶链反应（RT-PCR）检测RNA，采用酶联免疫吸附测定（ELISA）检测抗E2抗体。然后，对GBV-C/HGV RNA或抗E2抗体血液样本呈阳性的患者的精液和唾液样本的无细胞部分进行该病毒RNA存在情况的分析。此外，还检测了6例GBV-C/HGV阴性男性的精液样本和11例唾液样本。
33例患者中有11例（33.3%）血液样本经RT-PCR检测出GBV-C/HGV RNA，6例患者（18.2%）检测出E2包膜蛋白抗体。由于没有患者两种标志物均呈阳性，因此在所研究人群中GBV-C/HGV感染的总体患病率为51.5%。在血液中带有GBV-C/HGV标志物的17名男性中，有4名（均属于同性恋风险组）黏膜样本中GBV-C/HGV RNA呈阳性：其中2名在精液和唾液中均呈现基因组RNA，另外2名仅精液呈阳性。除1例唾液样本和1例精液样本外，所有GBV-C/HGV RNA呈阴性的黏膜部分均证实不存在PCR技术抑制剂。
这些结果证实了性接触感染HIV-1患者中GBV-C/HGV感染的高患病率，以及血液中带有该病毒标志物的男性精液和唾液中存在GBV-C/HGV RNA，这表明黏膜液可能是GBV-C/HGV感染传播的潜在来源。
