Hollingsworth R C, Jameson C L, Minton J E, Crowe M, Curran R, Rowe T, Grabowska A M, Pillay D, Irving W L, Ball J K
Division of Microbiology and Infectious Diseases, University of Nottingham, Queen's Medical Centre, UK.
J Med Virol. 1998 Dec;56(4):321-6. doi: 10.1002/(sici)1096-9071(199812)56:4<321::aid-jmv6>3.0.co;2-v.
Sequential paired samples of blood and seminal fluid were obtained from a cohort of 54 HIV-1-infected homosexual males. The prevalence of GBV-C/HGV RNA in the cell-free fractions of some of these patients was determined using reverse-transcription polymerase chain reaction (RT-PCR). To assess the effects of HIV-1 and HCV infection upon GBV-C/HGV RNA status, blood CD4 cell counts, HCV RNA status, and HIV-1 proviral DNA and viral RNA titres were also determined. GBV-C/HGV RNA was detected in 8/30 (27%) of the blood plasma samples obtained at the start of the study, and was present at a frequency of 14/64 (22%) in all the blood plasma samples tested. By contrast, GBV-C/HGV RNA was not detected in the 26 seminal fluid samples obtained at the start of the study, including 8 samples obtained from patients for which GBV-C/HGV RNA was detected in the corresponding blood sample. Of the samples tested for the presence of both GBV-C/HGV and HCV RNA, there was no evidence of coinfection. Although GBV-C/HGV RNA detection rates were significantly higher in individuals with blood CD4 cell counts greater than 200 cells per microlitre, there were no significant differences in the median blood CD4 cell counts or HIV-1 proviral DNA or viral RNA titres observed between the GBV-C/HGV-positive and -negative individuals. The failure to detect GBV-C/HGV RNA in seminal fluid samples obtained from this cohort would suggest that further studies need to be carried out to determine the roles of sexual transmission and of seminal fluid in GBV-C/HGV infection.
从54名感染HIV-1的同性恋男性队列中获取了血液和精液的序贯配对样本。使用逆转录聚合酶链反应（RT-PCR）测定了其中一些患者无细胞部分中GBV-C/HGV RNA的流行率。为了评估HIV-1和HCV感染对GBV-C/HGV RNA状态的影响，还测定了血液CD4细胞计数、HCV RNA状态以及HIV-1前病毒DNA和病毒RNA滴度。在研究开始时采集的30份血浆样本中，有8份（27%）检测到GBV-C/HGV RNA，在所有检测的血浆样本中，其出现频率为14/64（22%）。相比之下，在研究开始时采集的26份精液样本中未检测到GBV-C/HGV RNA，其中包括8份从相应血液样本中检测到GBV-C/HGV RNA的患者的样本。在检测GBV-C/HGV和HCV RNA共存的样本中，没有合并感染的证据。尽管血液CD4细胞计数大于每微升200个细胞的个体中GBV-C/HGV RNA检测率显著更高，但在GBV-C/HGV阳性和阴性个体之间，观察到的血液CD4细胞计数中位数、HIV-1前病毒DNA或病毒RNA滴度没有显著差异。从该队列中获得的精液样本未检测到GBV-C/HGV RNA，这表明需要进一步开展研究以确定性传播和精液在GBV-C/HGV感染中的作用。
