Rumyantseva G N, Voloshina D A, Kalunyants K Y, Grebeshova R N, Shain S S
Prikl Biokhim Mikrobiol. 1975 Jul-Aug;11(4):554-8.
The use of enzymic preparations of the cellulolytic and macerating effect was studied as applied to the isolation of diosgenine from rhizomes of Dioscorea caucasica Lypsky. The enzymic treatment of the steroid containing raw material prior to acid hydrolysis increased the yield of diosgenine by 30-48%. It is suggested that additional extraction of diosgenine takes place due to: 1) enzymic hydrolysis of structural polysaccharide components of the plant tissue and intercellular binding materials and 2) disintegration of glycoside bonds of saponins.
研究了具有纤维素分解和浸渍作用的酶制剂在从高加索薯蓣（Dioscorea caucasica Lypsky）根茎中分离薯蓣皂苷元方面的应用。在酸水解之前对含甾体原料进行酶处理，可使薯蓣皂苷元的产率提高30 - 48%。有人认为，薯蓣皂苷元的额外提取是由于：1）植物组织的结构多糖成分和细胞间结合物质的酶水解；2）皂苷糖苷键的分解。
