Khuchua G N, Stasiak A B
Prikl Biokhim Mikrobiol. 1975 Nov-Dec;11(6):914-21.
A method to determine S-methyl methionine (vitamin U) in plants has been devised. The method includes S-methyl methionine extraction extract purification on cationite KU-2 in the NH4+-form, S-methyl methionine eluation with 2n water solution of NH4OH from the cation exchange resin, eluate evaporation in vacuo, dissolution of the residue in a minimal amount of water acidified with hydrochloric acid, electrophoretic separation of S-methyl methionine from basic amino acids, ninhydrine development of the electrophoregram, methanol eluation of the spot corresponding to S-methyl methionine, and colorimetry of the solution at 506 mmu. Relative error is +/-2.5%.
已设计出一种测定植物中S-甲基蛋氨酸（维生素U）的方法。该方法包括在NH4+形式的阳离子交换树脂KU-2上进行S-甲基蛋氨酸提取和提取物纯化，用NH4OH的2n水溶液从阳离子交换树脂上洗脱S-甲基蛋氨酸，真空蒸发洗脱液，将残留物溶解在少量用盐酸酸化的水中，从碱性氨基酸中电泳分离S-甲基蛋氨酸，茚三酮显色电泳图谱，用甲醇洗脱与S-甲基蛋氨酸对应的斑点，并在506毫微米处对溶液进行比色测定。相对误差为±2.5%。
