Brunsch Christa, Sternstein Ina, Reinecke Peter, Bieniek Józef
Humboldt-University of Berlin, Agricultural and Horticultural Faculty, Institute of Animal Sciences, Department of Breeding Biology and Molecular Animal Breeding, Berlin, Germany.
J Appl Genet. 2002;43(1):85-91.
PIT1 was chosen as a candidate gene to investigate its associations with growth, meat quality and carcass composition traits in the pig. PIT1 is known as the pituitary-specific activator of the growth hormone in several mammals. Furthermore, PIT1 is a positive regulatory factor of prolactin and thyroid-stimulating hormone beta. PIT1 is a member of the POU-domain family of genes and is located on porcine chromosome 13. Two informative three-generation families of the University of Hohenheim were used for the presented investigations. The families were based on crosses of the European Wild boar (W) x Pietrain (P) and Meishan (M) x Pietrain (P). Each family included 310 F(2) animals. A RsaI (PCR) RFLP described by Yu et al. (1994) was used for genotyping the animals. Altogether over 50 parameters of growth, meat quality, carcass composition and stress susceptibility were evaluated concerning their associations with RsaI PCR-RFLP. The statistical model of association analyses was used including fixed effects of sex, family, PIT1 genotypes and covariate age at slaughter. Taking the significance level of p < 0.05 as the basis, fourteen traits of growth and carcass composition were associated with PIT1 genotypes in family W x P. Results from this study suggest that there are contributions of PIT1 gene to variations in the analysed performance traits in pigs. The influence of PIT1 genotypes could not be confirmed under the supposition of a genome-wide test limit.
选择PIT1作为候选基因，以研究其与猪的生长、肉质和胴体组成性状之间的关联。在几种哺乳动物中，PIT1被认为是生长激素的垂体特异性激活因子。此外，PIT1是催乳素和促甲状腺激素β的正调控因子。PIT1是POU结构域基因家族的成员，位于猪的13号染色体上。本研究使用了 Hohenheim大学的两个信息丰富的三代家系。这些家系基于欧洲野猪（W）×皮特兰（P）以及梅山猪（M）×皮特兰（P）的杂交。每个家系包括310只F(2)代动物。使用了Yu等人（1994年）描述的RsaI（PCR）RFLP对动物进行基因分型。总共评估了50多个关于生长、肉质、胴体组成和应激易感性的参数与RsaI PCR-RFLP的关联。关联分析的统计模型包括性别、家系、PIT1基因型和屠宰时协变量年龄的固定效应。以p < 0.05的显著性水平为基础，在W×P家系中，有14个生长和胴体组成性状与PIT1基因型相关。本研究结果表明，PIT1基因对猪的分析性能性状变异有贡献。在全基因组测试限度的假设下，无法证实PIT1基因型的影响。
