Jalil-Sarghale A, Moradi Shahrbabak M, Moradi Sharbabak H, Sadeghi M, Mura M C
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran,
Mol Biol Rep. 2014 Sep;41(9):5787-92. doi: 10.1007/s11033-014-3451-8. Epub 2014 Jun 26.
The pituitary-specific positive transcription factor 1 (POU1F1) gene has been the subject of many recent studies because of its important roles in growth and development of mammals. In this study, we investigated the single nucleotide polymorphisms (SNPs) at the third exon of POU1F1 gene and its association with growth and biometric traits and blood metabolites in two Iranian sheep breeds, Zel and Lori-Bakhtiari. Blood samples from 90 Lori-Bakhtiari and 90 Zel sheep were collected to extract DNA and the 295-bp fragment of the POU1F1 gene was amplified and the restriction fragment length polymorphism (RFLP) technique was adopted for genotyping. A SNP was identified in both Lori-Bakhtiari and Zel sheep breeds, which represents a non-synonymous single base mutation at restriction site for endonuclease AciI. The results revealed differential frequencies of alleles between the two studied breeds, where A allele was more frequent in Lori-Bakhtiari breed, while G allele was more frequent in Zel breed. When POU1F1 genotypes were tested, the animals with AA genotype had a higher weaning weight than those with GG genotype (p < 0.05), however there were not significant association between genotypes and birth weight, biometric traits (body length, body height, heart girth, thigh girth and abdominal girth) and blood metabolites (triglyceride and cholesterol) of the studied breeds (p > 0.05). These findings imply that the POU1F1 polymorphism may affect weaning weight, thus can be used as a molecular marker for this production trait.
垂体特异性正转录因子1（POU1F1）基因因其在哺乳动物生长发育中的重要作用，成为近期众多研究的对象。在本研究中，我们调查了POU1F1基因第三外显子的单核苷酸多态性（SNP）及其与两个伊朗绵羊品种Zel和Lori - Bakhtiari的生长、生物特征性状及血液代谢产物的关联。采集了90只Lori - Bakhtiari绵羊和90只Zel绵羊的血样以提取DNA，扩增了POU1F1基因的295 bp片段，并采用限制性片段长度多态性（RFLP）技术进行基因分型。在Lori - Bakhtiari和Zel绵羊品种中均鉴定出一个SNP，它代表了内切酶AciI限制位点处的一个非同义单碱基突变。结果显示，两个研究品种之间等位基因频率存在差异，其中A等位基因在Lori - Bakhtiari品种中更常见，而G等位基因在Zel品种中更常见。对POU1F1基因型进行检测时，AA基因型的动物断奶体重高于GG基因型的动物（p < 0.05），然而，基因型与所研究品种的出生体重、生物特征性状（体长、体高、胸围、大腿围和腹围）及血液代谢产物（甘油三酯和胆固醇）之间无显著关联（p > 0.05）。这些发现表明，POU1F1多态性可能影响断奶体重，因此可作为该生产性状的分子标记。
