Laboratory of Animal Genetic and Breeding, Institute of Animal Genetics and Breeding, Sichuan Agriculture University, Xingkang Road 46#, Ya'an, Sichuan 625014, People's Republic of China.
Mol Biol Rep. 2012 Aug;39(8):8363-71. doi: 10.1007/s11033-012-1686-9. Epub 2012 Jun 22.
POU1F1 is an essential factor that regulates the development and reproduction of animal. The objective of the current research was to screen for polymorphism, expression of POU1F1 and their association with carcass quality traits. A total of 126 Erlang mountainous chickens from two strains (SD02 and SD03) were employed for testing. Seventeen single nucleotide polymorphisms (SNPs) were detected, but only two SNPs (g.96217999 T > C and g.96219442 C > T) were associated with carcass quality traits. In SD03 chicken, g.96217999 T > C genotypes were significantly associated with body weight (BW), carcass weight (CW), eviscerated weight (EW), and semi-eviscerated weight (SEW; P < 0.05), and was highly significantly associated with breast muscle weight (BMW) and abdominal fat weight (AW; P < 0.01). g.96219442 C > T was significantly associated with BW, EW, SEW (P < 0.05). However, these two SNPs were not significantly associated with any carcass traits in SD02 chicken. Diplotypes showed that in SD03 chicken, the haplotype [C: C] was the most favorable haplotype because it was associated with higher BW, CW, SEW, EW, BMW, and AW (P < 0.05). On the contrary, haplotype [T: T] was associated with lower carcass quality traits (P < 0.01). In addition, qRT-PCR revealed that at 13 weeks, the POU1F1 mRNA expression was significantly higher in breast muscle of cock compared to that of hens (P < 0.05), whereas there was no significant correlation between POU1F1 expression and carcass traits. These results suggested that POU1F1 could be a potential candidate gene for carcass traits in chicken.
POU1F1 是调节动物发育和繁殖的必需因子。本研究旨在筛选多态性、POU1F1 的表达及其与胴体品质性状的关系。选用两个品系（SD02 和 SD03）的 126 只二郎山山地鸡进行试验。共检测到 17 个单核苷酸多态性（SNP），但只有两个 SNP（g.96217999T>C 和 g.96219442C>T）与胴体品质性状相关。在 SD03 鸡中，g.96217999T>C 基因型与体重（BW）、胴体重（CW）、屠体重（EW）和半屠体重（SEW）显著相关（P<0.05），与胸肌重（BMW）和腹脂重（AW）高度显著相关（P<0.01）。g.96219442C>T 与 BW、EW、SEW 显著相关（P<0.05）。然而，这两个 SNP 与 SD02 鸡的任何胴体性状均无显著相关性。单体型分析表明，在 SD03 鸡中，[C:C]单体型是最有利的单体型，因为它与更高的 BW、CW、SEW、EW、BMW 和 AW 相关（P<0.05）。相反，[T:T]单体型与较低的胴体品质性状相关（P<0.01）。此外，qRT-PCR 显示，在 13 周龄时，公鸡的胸肌中 POU1F1mRNA 的表达明显高于母鸡（P<0.05），但 POU1F1 表达与胴体性状之间无显著相关性。这些结果表明，POU1F1 可能是鸡胴体性状的一个潜在候选基因。
