Prabhu N, Babu J Sarat Chandra, Sundaram S
Department of Chemical Engineering, Regional Engineering College, Tiruchirappalli-620 015, India.
Biomed Sci Instrum. 2002;38:495-500.
The production of L-Glutamic acid has been studied using coimmobilized whole cells of pseudomonas reptilivora and micrococcus glutamicus in a two litre Tokyo Rikakikai fermentor using glucose as selected production medium. The process was carried out at an optimum temperature of 32 degree Celsius and a pH of 7.2. The progress of the reaction was recorded using Dr. Ingold fluorosensor. The effect of initial substrate concentration, speed of agitation, volume ofcalcium alginate beads and aeration rate on the yield of glutamic acid has been investigated. It has been found that the acid production increases exponentially with substrate concentration, and mass transfer co-efficient varied linearly with aeration rate. The kinetic parameters also had been estimated.
利用东京理化器械公司的两升发酵罐，以葡萄糖为选定的生产培养基，对固定化的爬行假单胞菌和谷氨酸微球菌全细胞生产L-谷氨酸进行了研究。该过程在32摄氏度的最佳温度和pH值7.2下进行。使用英戈尔德博士荧光传感器记录反应进程。研究了初始底物浓度、搅拌速度、海藻酸钙珠体积和通气速率对谷氨酸产量的影响。已发现酸产量随底物浓度呈指数增加，传质系数随通气速率呈线性变化。还估算了动力学参数。
