Sundaram S, Chopra D, Krithiga S, Damodaran V
Department of Chemical Engineering, Regional Engineering College, Tiruchirappalli, India.
Biomed Sci Instrum. 1994;30:117-25.
Lactose has been fermented in a Tokyo Rikakikai Fermentor using two percent seeding at various temperatures. The progress of the reaction was followed by measuring the fluorescent signal due to NADH with a Dr. Ingold (Switzerland) fluorosensor which has an excitation wave length of 360 nm and measurement wavelength of 450 nm. The optimum temperature for this fermentation reaction is 34 degree celsius. At this temperature while biomass growth rate and final biomass concentration are a maximum the time taken to reach the final biomass concentration and lag time are a minimum. The fluorescent voltage vs time data fitted a first order plus dead time model with an error of less than one percent. The present work is in good agreement with earlier work on glucose and lactose fermentation with one percent seeding. The increase in seeding from one to two percent has improved the optimum parameters studied. Further work on higher seeding concentration and higher order models is in progress.
乳糖已在东京理化器械株式会社发酵罐中，于不同温度下以2%的接种量进行发酵。反应进程通过使用瑞士英戈尔德博士荧光传感器测量由于NADH产生的荧光信号来跟踪，该传感器的激发波长为360纳米，测量波长为450纳米。该发酵反应的最佳温度为34摄氏度。在此温度下，生物量生长速率和最终生物量浓度达到最大值，而达到最终生物量浓度所需的时间和延迟时间则为最小值。荧光电压与时间数据符合一阶加死时间模型，误差小于1%。目前的工作与早期关于1%接种量的葡萄糖和乳糖发酵的工作高度一致。接种量从1%增加到2%改善了所研究的最佳参数。关于更高接种浓度和高阶模型的进一步研究正在进行中。
