Sundaram S, Sailaja D, Kalpana N
Department of Chemical Engineering, Regional Engineering College, Tiruchirappalli, India.
Biomed Sci Instrum. 1997;34:175-80.
Dextrose with differing amounts of xylose (mixed substrate medium) has been fermented at 28 degree Celsius with sacchromyces cerevisiae (Baker's Yeast) as seeding. The progress of the reaction was recorded by measuring the fluorescent signal due to intracellular reduced nicotinamide adenine di nucleotide (NADH) present in the cells with a Dr. Ingold (Switzerland) fluorosensor which has an excitation wavelength of 360 nm and measurement wavelength of 450 nm. The concentration of xylose in the xylose-dextrose feed was varied from 0.7% to 5.0% by weight. The optimum concentration of xylose at which the production of alcohol was a maximum was found to be 3.4 percent xylose. The fluorescent voltage data for different concentration of xylose fitted a first order model with an average absolute deviation of less than one percent. Development of this model is useful in design of model predictive controllers.
含有不同量木糖的葡萄糖（混合底物培养基）在28摄氏度下用酿酒酵母（面包酵母）作为种子进行发酵。通过使用英戈尔德博士（瑞士）荧光传感器测量细胞内存在的细胞内还原型烟酰胺腺嘌呤二核苷酸（NADH）产生的荧光信号来记录反应进程，该传感器的激发波长为360nm，测量波长为450nm。木糖 - 葡萄糖进料中木糖的浓度按重量计从0.7％变化到5.0％。发现酒精产量最高时木糖的最佳浓度为3.4％木糖。不同木糖浓度的荧光电压数据拟合一阶模型，平均绝对偏差小于1％。该模型的开发对模型预测控制器的设计很有用。
