Skórska Czesława, Krysińska-Traczyk Ewa, Milanowski Janusz, Cholewa Grazyna, Sitkowska Jolanta, Góra Anna, Dutkiewicz Jacek
Department of Occupational Biohazards, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
Ann Agric Environ Med. 2002;9(1):91-7.
The aim of this work was to determine the reactivity of furniture factory workers to microbial allergens associated with wood dust. Allergological examinations by skin and precipitin tests were performed in 48 workers employed in a factory producing furniture from fibreboards and chipboards, and in 32 healthy urban dwellers not exposed to organic dusts (referents). The skin test was performed by the intradermal method with the saline extracts of the cultures of 3 microbial species (Rahnella sp., Arthrobacter globiformis, Aspergillus fumigatus) associated with wood dust. Skin reactions were recorded after 20 minutes, 8 hours and 24 hours and graded 1-4, depending on the diameter of the reaction. The agar-gel test for the presence of precipitins in serum was performed with the extracts of 15 microbial isolates. The furniture factory workers showed a high skin response to the extracts of environmental microbes. The frequency of early grade 2 reactions (diameter 10 mm) to the extract of Rahnella sp. was 64.6% among furniture workers, being significantly higher (p < 0.001) compared to reference group (18.7%). High frequencies of grade 2 reactions in furniture workers were also found with the extracts of A. globiformis and A. fumigatus (52.1% and 62.5%, respectively). The frequencies of grade 2 delayed (after 8 h) and late (after 24 h) reactions to Rahnella sp. in furniture workers were non-specifically high (97.9%/93.7%) while the response rates to A. globiformis and A. fumigatus were much lower (10.4%/25.0%, and 4.2%/37.5%, respectively). In agar-gel test for detection of precipitins, in most cases very low percentages of positive reactions (0-2.1%) were noted in furniture factory workers. The only exception was a high percentage of positive reactions (27.1%) to the antigen of Pseudomonas maltophilia, which was significantly greater in furniture workers compared to the reference group (p < 0.01). The obtained results suggest that early allergic reactions to microorganisms associated with wood dust are common among workers of furniture industry, which may increase a potential risk of work-related disease in this occupational group.
这项工作的目的是确定家具厂工人对与木尘相关的微生物过敏原的反应性。对一家生产纤维板和刨花板家具的工厂的48名工人以及32名未接触有机粉尘的健康城市居民（对照）进行了皮肤和沉淀素试验的变态反应学检查。皮肤试验采用皮内法，使用与木尘相关的3种微生物（拉恩菌属、球形节杆菌、烟曲霉）培养物的盐水提取物。在20分钟、8小时和24小时后记录皮肤反应，并根据反应直径分为1 - 4级。用15种微生物分离物的提取物进行琼脂凝胶试验以检测血清中沉淀素的存在。家具厂工人对环境微生物提取物表现出较高的皮肤反应。家具工人中对拉恩菌属提取物的早期2级反应（直径10毫米）频率为64.6%，与对照组（18.7%）相比显著更高（p < 0.001）。在家具工人中，对球形节杆菌和烟曲霉提取物也发现了较高频率的2级反应（分别为52.1%和62.5%）。家具工人中对拉恩菌属的2级延迟（8小时后）和晚期（24小时后）反应频率非特异性地高（97.9%/93.7%），而对球形节杆菌和烟曲霉的反应率则低得多（分别为10.4%/25.0%和4.2%/37.5%）。在检测沉淀素的琼脂凝胶试验中，在大多数情况下，家具厂工人的阳性反应百分比非常低（0 - 2.1%）。唯一的例外是对嗜麦芽窄食单胞菌抗原的阳性反应百分比很高（27.1%），家具工人中的这一比例与对照组相比显著更高（p < 0.01）。所得结果表明，家具行业工人中对与木尘相关微生物的早期过敏反应很常见，这可能会增加该职业群体患职业病的潜在风险。
