Mukabana W R, Takken W, Seda P, Killeen G F, Hawley W A, Knols B G J
International Centre of Insect Physiology and Ecology (ICIPE), PO Box 30772, Nairobi, Kenya.
Bull Entomol Res. 2002 Jun;92(3):233-9. doi: 10.1079/BER2002164.
The success of distinguishing blood meal sources of Anopheles gambiae Giles through deoxyribonucleic acid (DNA) profiling was investigated by polymerase chain reaction (PCR) amplification at the TC-11 and VWA human short tandem repeats (STR) loci. Blood meal size and locus had no significant effect on the success of amplifying human DNA from blood meals digested for 0, 8, 16, 24 and 32 h (P = 0.85 and 0.26 respectively). However, logistic regression found a significant negative relationship between time since ingestion and the success probability of obtaining positive PCR products among meals digested for between 8 and 32 h (P = 0.001). Approximately 80% of fresh blood meals were successfully profiled. After 8 h, the proportion of blood meals that could be successfully profiled decreased slowly with time after ingestion, dropping to below 50% after approximately 15 h. There was no significant difference in the success of amplifying human DNA from blood meals of mosquitoes killed at time 0 and 8 h after ingestion (P = 0.272).
通过聚合酶链反应（PCR）扩增在TC-11和VWA人类短串联重复序列（STR）位点，研究了利用脱氧核糖核酸（DNA）分析鉴定冈比亚按蚊（Anopheles gambiae Giles）血餐来源的成功率。血餐大小和位点对从消化0、8、16、24和32小时的血餐中扩增人类DNA的成功率没有显著影响（P值分别为0.85和0.26）。然而，逻辑回归发现，在消化8至32小时的血餐中，摄入后时间与获得阳性PCR产物的成功概率之间存在显著的负相关关系（P = 0.001）。大约80%的新鲜血餐成功进行了分析。8小时后，随着摄入后时间的推移，能够成功分析的血餐比例缓慢下降，大约15小时后降至50%以下。在摄入后0小时和8小时杀死的蚊子血餐中扩增人类DNA的成功率没有显著差异（P = 0.272）。
