Häkkinen H M, Franssila K, Kulonen E
Scand J Clin Lab Invest. 1975 Dec;35(8):753-65. doi: 10.3109/00365517509095807.
Rats drank ethanol, on the average 1.20 g/100 g body weight, for various periods up to nearly 300 days. Experimental variables included a high-fat, low-protein diet, administration of additional ethanol by stomach tube, and CCl4 injections instead of ethanol. Growth was retarded by all the variables, especially by the high-fat, low-protein diet. The specific histological finding in the ethanol groups was the presence of Mallory bodies. Significant increase in total liver lipids was caused by ethanol, and rapid fat accumulation, inflammatory changes, and even fibrosis and cirrhosis by the high-fat, low-protein diet and the CCl4 injections. Ethanol raised the concentrations of collagen and soluble protein in the liver; the collagen content was increased also by the high-fat, low-protein diet and the CCl4 injections. The incorporation of proline to collagen was stimulated in incubated liver slices from both ethanol-treated and high-fat, low-protein-fed rats. These treatments also increased the concentration of free proline in the liver, thus augmenting the protein synthesis in fibroblasts.
大鼠饮用乙醇，平均摄入量为1.20克/100克体重，持续不同时间段长达近300天。实验变量包括高脂低蛋白饮食、通过胃管额外给予乙醇以及注射四氯化碳而非乙醇。所有这些变量都会阻碍生长，尤其是高脂低蛋白饮食。乙醇组的特定组织学发现是存在马洛里小体。乙醇导致肝脏总脂质显著增加，而高脂低蛋白饮食和注射四氯化碳则导致快速的脂肪堆积、炎症变化，甚至纤维化和肝硬化。乙醇提高了肝脏中胶原蛋白和可溶性蛋白的浓度；高脂低蛋白饮食和注射四氯化碳也增加了胶原蛋白含量。在经乙醇处理的大鼠和高脂低蛋白喂养的大鼠的肝脏切片培养物中，脯氨酸掺入胶原蛋白的过程受到刺激。这些处理还增加了肝脏中游离脯氨酸的浓度，从而增强了成纤维细胞中的蛋白质合成。
