Wyatt Kenneth M, Wyatt Gemma L
Murdoch University Veterinary Hospital, Department of Veterinary Clinical Science, Murdoch University, Perth, Australia.
J Am Vet Med Assoc. 2002 Jun 15;220(12):1805-6. doi: 10.2460/javma.2002.220.1805.
To determine accuracy of a manual technique for detection of neutropenia and thrombocytopenia in dogs receiving chemotherapy.
Masked prospective study.
11 dogs treated with chemotherapy for neoplasia.
124 blood samples from dogs being treated with chemotherapy for various neoplasms were processed through an automated cell counter, and results were compared with those obtained by use of a rapid manual technique for estimating neutrophil and platelet concentrations to determine whether the manual technique could accurately detect dogs with neutropenia or thrombocytopenia.
By use of automated techniques, neutropenia (< 3,000 cells/microl) was detected in 17 of 124 blood samples, and thrombocytopenia (< 100,000 platelets/microl) was detected in 3 of 124 blood samples. The manual technique correctly identified 16 of 17 (94%) blood samples with neutropenia, with a specificity of 92% (98/107). The manual technique correctly identified 3 of 3 (100%) blood samples with thrombocytopenia, with specificity of 94% (114/121).
Manual estimates of neutrophil and platelet counts are sensitive and specific; however, a full differential cell count is still preferable.
确定一种手动技术检测接受化疗犬中性粒细胞减少和血小板减少的准确性。
盲法前瞻性研究。
11只接受化疗治疗肿瘤的犬。
对124份接受各种肿瘤化疗犬的血样通过自动血细胞计数器进行处理，并将结果与使用快速手动技术估算中性粒细胞和血小板浓度获得的结果进行比较，以确定该手动技术能否准确检测出中性粒细胞减少或血小板减少的犬。
使用自动技术，在124份血样中有17份检测到中性粒细胞减少（<3,000个细胞/微升），在124份血样中有3份检测到血小板减少（<100,000个血小板/微升）。手动技术正确识别出17份中性粒细胞减少血样中的16份（94%），特异性为92%（98/107）。手动技术正确识别出3份血小板减少血样中的3份（100%），特异性为94%（114/121）。
手动估算中性粒细胞和血小板计数具有敏感性和特异性；然而，进行完整的血细胞分类计数仍更为可取。
