Zapala Matthew A, Lockhart Daniel J, Pankratz Daniel G, Garcia Anthony J, Barlow Carrolee, Lockhart David J
The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Genome Biol. 2002;3(6):SOFTWARE0001. doi: 10.1186/gb-2002-3-6-software0001. Epub 2002 May 23.
Two HTML-based programs were developed to analyze and filter gene-expression data: 'Bullfrog' for Affymetrix oligonucleotide arrays and 'Spot' for custom cDNA arrays. The programs provide intuitive data-filtering tools through an easy-to-use interface. A background subtraction and normalization program for cDNA arrays was also built that provides an informative summary report with data-quality assessments. These programs are freeware to aid in the analysis of gene-expression results and facilitate the search for genes responsible for interesting biological processes and phenotypes.
开发了两个基于HTML的程序来分析和筛选基因表达数据:用于Affymetrix寡核苷酸阵列的“牛蛙”程序和用于定制cDNA阵列的“Spot”程序。这些程序通过易于使用的界面提供直观的数据筛选工具。还构建了一个用于cDNA阵列的背景扣除和标准化程序,该程序提供带有数据质量评估的信息丰富的总结报告。这些程序是免费软件,有助于分析基因表达结果,并便于寻找与有趣的生物学过程和表型相关的基因。