Nagaralli B S, Seetharamappa J, Melwanki M B
Department of Chemistry, Karnatak University, 580 003, Dharwad, India.
J Pharm Biomed Anal. 2002 Jul 31;29(5):859-64. doi: 10.1016/s0731-7085(02)00210-8.
Two simple, sensitive and accurate spectrophotometric methods have been proposed for the determination of amoxycillin (AMX), ciprofloxacin (CPF) and piroxicam (PIR) in pure and pharmaceutical preparations. The methods are based on the measurement of absorbances of tris(o-phenanthroline) iron(II) [method A] and tris (bipyridyl) iron(II) [method B] complexes at 510 and at 522 nm, respectively. Reaction conditions have been optimized to obtain coloured complexes of higher sensitivity and longer stability. The absorbances were found to increase linearly with increase in concentrations of AMX, CPF and PIR which were corroborated by correlation coefficient values. The complexes obeyed Beer's law over the concentration range of 0.06-5.2, 0.04-7.2 and 0.2-6.5 microg ml(-1) for AMX, CPF and PIR, respectively, in method A, and of 0.05-8.5, 0.05-9.0 and 0.05-6.5 microg ml(-1) for AMX, CPF and PIR, respectively, in method B. The developed methods have been successfully applied for the determination of AMX, CPF and PIR in bulk drugs and pharmaceutical formulations. The common excipients and additives did not interfere in their determinations. The results obtained by the proposed methods have been statistically compared by means of Student t-test and by the variance ratio F-test.
已提出两种简单、灵敏且准确的分光光度法，用于测定纯品及药物制剂中的阿莫西林（AMX）、环丙沙星（CPF）和吡罗昔康（PIR）。这两种方法分别基于测定三（邻菲啰啉）铁（II）[方法A]和三（联吡啶）铁（II）[方法B]配合物在510 nm和522 nm处的吸光度。已对反应条件进行优化，以获得灵敏度更高且稳定性更长的有色配合物。发现吸光度随AMX、CPF和PIR浓度的增加呈线性增加，相关系数值证实了这一点。在方法A中，AMX、CPF和PIR的配合物分别在0.06 - 5.2、0.04 - 7.2和0.2 - 6.5 μg ml⁻¹的浓度范围内符合比尔定律；在方法B中，AMX、CPF和PIR的配合物分别在0.05 - 8.5、0.05 - 9.0和0.05 - 6.5 μg ml⁻¹的浓度范围内符合比尔定律。所开发的方法已成功应用于原料药和药物制剂中AMX、CPF和PIR的测定。常见的辅料和添加剂在其测定中不产生干扰。通过学生t检验和方差比F检验对所提出方法获得的结果进行了统计学比较。
