Microimmunoassay using a protein chip: optimizing conditions for protein immobilization.
作者信息
Seong Seung-Yong
机构信息
Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Disease, Seoul National University Medical Research Center, Chongno-gu, Seoul 110-799, Republic of Korea.
出版信息
Clin Diagn Lab Immunol. 2002 Jul;9(4):927-30. doi: 10.1128/cdli.9.4.927-930.2002.
Abstract
Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.
摘要
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