Cho Jae Youl, Kim Ae Ra, Yoo Eun Sook, Baik Kyong Up, Park Myung Hwan
Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London Medical School, London, UK.
Planta Med. 2002 Jun;68(6):497-500. doi: 10.1055/s-2002-32556.
We have examined the immunosuppressive effects of representative ginsenosides (Rb1, Rb2, Re and Rg1) from Panax ginseng C. A. Meyer on CD4+ and CD8+ lymphocyte proliferation. Ginsenosides differentially modulated lymphocyte proliferation induced by concanavalin A (Con A), lipopolysaccharide (LPS), phytohemaglutinin (PHA) and interleukin-2 (IL-2). Thus, Rb1 and Re significantly enhanced Con A-induced lymphocyte proliferation, whereas Rg1 did not affect the proliferation. Interestingly, however, Rb2 strongly blocked Con A, LPS and PHA-induced lymphocyte proliferation with the IC50 values of 21.8, 29.0 and 24.0 microM, respectively. Moreover, Rb2 inhibited Con A-stimulated IL-2 production with an IC 50 of 13.3 microM. In the IL-2-stimulated CD8+ T cell (CTLL-2) proliferation assay, Re and Rg1 showed strong suppressive effects with IC50 values of 57.5 and 64.7 microM, respectively. In contrast, neither Rb1 nor Rb2 did inhibit CTLL-2 cell proliferation at tested concentrations. These results suggest that ginsenosides from P. ginseng may modulate lymphocyte proliferation in a different manner.
我们研究了人参（Panax ginseng C. A. Meyer）中代表性人参皂苷（Rb1、Rb2、Re和Rg1）对CD4 +和CD8 +淋巴细胞增殖的免疫抑制作用。人参皂苷对伴刀豆球蛋白A（Con A）、脂多糖（LPS）、植物血凝素（PHA）和白细胞介素-2（IL-2）诱导的淋巴细胞增殖有不同的调节作用。因此，Rb1和Re显著增强Con A诱导的淋巴细胞增殖，而Rg1对增殖无影响。然而，有趣的是，Rb2强烈阻断Con A、LPS和PHA诱导的淋巴细胞增殖，其IC50值分别为21.8、29.0和24.0微摩尔。此外，Rb2抑制Con A刺激的IL-2产生，IC50为13.3微摩尔。在IL-2刺激的CD8 + T细胞（CTLL-2）增殖试验中，Re和Rg1表现出强烈的抑制作用，IC50值分别为57.5和64.7微摩尔。相比之下，在测试浓度下，Rb1和Rb2均未抑制CTLL-2细胞增殖。这些结果表明，人参中的人参皂苷可能以不同方式调节淋巴细胞增殖。
