Carrell Douglas T, Cartmill Deborah, Jones Kirtly P, Hatasaka Harry H, Peterson C Matthew
Division of Urology and Department of Obstetrics and Gynecology, of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Fertil Steril. 2002 Jul;78(1):16-21. doi: 10.1016/s0015-0282(02)03179-5.
To evaluate variability in donor semen quality between seven commercial donor sperm banks, within sperm banks, and between intracervical insemination and intrauterine insemination.
Prospective, randomized, blind evaluation of commercially available donor semen samples.
An academic andrology laboratory.
PATIENT(S): Seventy-five cryopreserved donor semen samples were evaluated.
INTERVENTION(S): Samples were coded, then blindly evaluated for semen quality.
MAIN OUTCOME MEASURE(S): Standard semen quality parameters, including concentration, motility parameters, World Health Organization criteria morphology, and strict criteria morphology.
RESULT(S): Significant differences were observed between donor semen banks for most semen quality parameters analyzed in intracervical insemination samples. In general, the greatest variability observed between banks was in percentage progressive sperm motility (range, 8.8 +/- 5.8 to 42.4 +/- 5.5) and normal sperm morphology (strict criteria; range, 10.1 +/- 3.3 to 26.6 +/- 4.7). Coefficients of variation within sperm banks were generally high.
CONCLUSION(S): These data demonstrate the variability of donor semen quality provided by commercial sperm banks, both between banks and within a given bank. No relationship was observed between the size or type of sperm bank and the degree of variability. The data demonstrate the lack of uniformity in the criteria used to screen potential semen donors and emphasize the need for more stringent screening criteria and strict quality control in processing samples.
评估7家商业供精库之间、各供精库内部以及宫颈内人工授精和宫腔内人工授精之间供精质量的差异。
对市售供精样本进行前瞻性、随机、盲法评估。
一个学术男科学实验室。
对75份冷冻供精样本进行评估。
对样本进行编码，然后对精液质量进行盲法评估。
标准精液质量参数，包括浓度、活力参数、世界卫生组织标准形态学和严格标准形态学。
在宫颈内人工授精样本中分析的大多数精液质量参数方面，各供精库之间存在显著差异。一般来说，各供精库之间观察到的最大差异在于精子前向运动百分比（范围为8.8±5.8至42.4±5.5）和正常精子形态（严格标准；范围为10.1±3.3至26.6±4.7）。各供精库内部的变异系数普遍较高。
这些数据表明商业供精库提供的供精质量存在差异，包括各供精库之间以及同一供精库内部。未观察到供精库的规模或类型与变异程度之间存在关联。数据表明筛选潜在供精者所用标准缺乏一致性，并强调在处理样本时需要更严格的筛选标准和严格的质量控制。
