Tan Xian-Jie, Lang Jing-He, Liu Dong-Yuan, Shen Keng, Leng Jin-Hua, Zhu Lan
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Fertil Steril. 2002 Jul;78(1):148-53. doi: 10.1016/s0015-0282(02)03187-4.
To investigate the expression of vascular endothelial growth factor (VEGF) mRNA and thrombospondin-1 (TSP-1) mRNA in endometriosis.
Molecular studies in human tissue.
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China.
PATIENT(S): Patients undergoing laparoscopy for infertility or other benign gynecologic conditions.
INTERVENTION(S): Biopsies were taken from endometriotic lesions (red peritoneal lesion, ovarian endometrioma, and unterosacral ligament nudule) and eutopic endometrium during laparoscopy.
MAIN OUTCOME MEASURE(S): mRNA expression from endometriotic lesion and eutopic endometrium was analyzed by reverse transcriptase polymerase chain reaction (PCR) and Northern blotting.
RESULT(S): Among the endometriotic lesions, red peritoneal lesions expressed higher levels of VEGF mRNA and lower levels of TSP-1 mRNA, whereas ovarian endometrioma expressed lower levels of VEGF mRNA and higher levels of TSP-1 mRNA. Eutopic endometrium of women with endometriosis had higher expression levels of VEGF mRNA and lower expression levels of TSP-1 mRNA than that of women without endometriosis.
CONCLUSION(S): The expression of VEGF and TSP-1 in endometriotic lesions appears to be associated with the extent of their neovascularization. The imbalance in expression of VEGF and TSP-1 in the endometrium may play a role in the development of endometriosis.
研究血管内皮生长因子（VEGF）mRNA和血小板反应蛋白-1（TSP-1）mRNA在子宫内膜异位症中的表达。
人体组织分子研究。
中国医学科学院北京协和医学院北京协和医院妇产科。
因不孕或其他良性妇科疾病接受腹腔镜检查的患者。
在腹腔镜检查期间，从子宫内膜异位症病变（红色腹膜病变、卵巢子宫内膜异位囊肿和子宫骶韧带结节）及在位内膜取活检组织。
采用逆转录聚合酶链反应（PCR）和Northern印迹法分析子宫内膜异位症病变组织及在位内膜的mRNA表达。
在子宫内膜异位症病变中，红色腹膜病变VEGF mRNA表达水平较高，TSP-1 mRNA表达水平较低；而卵巢子宫内膜异位囊肿VEGF mRNA表达水平较低，TSP-1 mRNA表达水平较高。子宫内膜异位症患者的在位内膜VEGF mRNA表达水平高于无子宫内膜异位症的女性，TSP-1 mRNA表达水平低于无子宫内膜异位症的女性。
VEGF和TSP-1在子宫内膜异位症病变中的表达似乎与其新生血管形成程度有关。子宫内膜中VEGF和TSP-1表达失衡可能在子宫内膜异位症的发生发展中起作用。
