Pucci-Minafra Ida, Fontana Simona, Cancemi Patrizia, Alaimo Giuseppina, Minafra Salvatore
University of Palermo, Center of Experimental Oncobiology, Department of Cell Biology and Development, Institute of Histology and Embryology, Palermo, Italy.
Ann N Y Acad Sci. 2002 Jun;963:122-39. doi: 10.1111/j.1749-6632.2002.tb04103.x.
Breast cancer is one of the leading causes of death from cancer among women in western countries. The different types of breast cancer are grouped into invasive and noninvasive forms. Among the invasive types, ductal infiltrating carcinoma (DIC) is the most common and aggressive form. Using an in vitro model consisting of a DIC-derived cell line (8701-BC) and a nontumoral mammary epithelial cell line (HB2), we used the proteomics approach to search for homology and differences in protein expression patterns between tumoral and nontumoral phenotypes. Within an analysis window comprising 1,750 discernible spots we have currently catalogued 140 protein spots of potential interest. Fifty-eight of them were identified by gel matching with reference maps, immunodetection, or N-terminal microsequencing and classified into four functional groups. Twelve proteins were found differentially expressed in two cell lines: four were uniquely present in the neoplastic cell proteome and eight in epithelial cells. In addition, 53 proteins displayed different relative expression levels between the two cell lines, that is, 44 were more elevated in cancer cells and 9 in HB2 cells. Among proteins with greater relative abundance in cancer cells we identified glycolytic enzymes (or their isoforms), which may indicate that the known metabolic dysregulation in cancer can reflect oncogenic-related defects of glycolytic gene expression.
乳腺癌是西方国家女性癌症死亡的主要原因之一。不同类型的乳腺癌分为浸润性和非浸润性。在浸润性类型中，导管浸润癌（DIC）是最常见且侵袭性最强的形式。我们使用由DIC衍生的细胞系（8701 - BC）和非肿瘤性乳腺上皮细胞系（HB2）组成的体外模型，采用蛋白质组学方法来寻找肿瘤和非肿瘤表型之间蛋白质表达模式的同源性和差异。在一个包含1750个可分辨斑点的分析窗口内，我们目前已编目了140个潜在感兴趣的蛋白质斑点。其中58个通过与参考图谱的凝胶匹配、免疫检测或N端微测序进行了鉴定，并分为四个功能组。发现有12种蛋白质在两种细胞系中差异表达：4种仅存在于肿瘤细胞蛋白质组中，8种存在于上皮细胞中。此外，53种蛋白质在两种细胞系之间显示出不同的相对表达水平，即44种在癌细胞中表达更高，9种在HB2细胞中表达更高。在癌细胞中相对丰度较高的蛋白质中，我们鉴定出了糖酵解酶（或其同工型），这可能表明癌症中已知的代谢失调可反映糖酵解基因表达的致癌相关缺陷。
