Kerley Simon J, Norgaard Claus, Leach John E, Christiansen Jøergen L, Huyghe Christian, Römer Peter
IACR-Rothamsted, Harpenden, Hertfordshire, UK.
Ann Bot. 2002 Mar;89(3):341-9. doi: 10.1093/aob/mcf046.
European cultivars of white lupin (Lupinus albus L.) grow poorly in limed or calcareous soils. However, Egyptian genotypes are grown successfully in highly calcareous soil and show no stress symptoms. To examine their physiological responses to alkaline soil and develop potential screens for tolerance, three experiments were conducted in limed and non-limed (neutral pH) soil. Measurements included net CO2 uptake, and the partitioning of Fe2+ and Fe3+ and soluble and insoluble Ca in stem and leaf tissue. Intolerant plants showed clear symptoms of stress, whereas stress in the Egyptian genotypes and in L pilosus Murr. (a tolerant species) was less marked. Only the intolerant plants became chlorotic and this contributed to their reduced net CO2 uptake in the limed soil. In contrast, Egyptian genotypes and L pilosus showed no change in net CO2 uptake between the soils. The partitioning of Ca and Fe either resulted from the stress responses, or was itself a stress response. L pilosus and some Egyptian genotypes differed in soluble Ca concentrations compared with the intolerant cultivars, although no significant difference was apparent in the Ca partitioning of the Egyptian genotype Giza 1. In a limed soil, Giza 1 maintained its stem Fe3+ concentration at a level comparable with that of plants grown in non-limed soil, whereas stem [Fe3+] of an intolerant genotype increased. Gizal increased the percentage of plant Fe that was Fe2+ in its leaf tissue under these conditions; that of the intolerant genotype was reduced. The potential tolerance of the Egyptian genotypes through these mechanisms and the possibility of nutritional-based screens are discussed.
欧洲白羽扇豆（Lupinus albus L.）品种在施用石灰的或石灰性土壤中生长不良。然而，埃及基因型在高石灰性土壤中能成功生长且无胁迫症状。为研究它们对碱性土壤的生理响应并开发潜在的耐受性筛选方法，在施用石灰的和未施用石灰的（中性pH）土壤中进行了三项实验。测量指标包括净二氧化碳吸收量，以及茎和叶组织中Fe2+和Fe3+以及可溶性和不溶性钙的分配情况。不耐受的植株表现出明显的胁迫症状，而埃及基因型和柔毛羽扇豆（L pilosus Murr.，一个耐受品种）的胁迫则不那么明显。只有不耐受的植株出现黄化现象，这导致它们在施用石灰的土壤中净二氧化碳吸收量减少。相比之下，埃及基因型和柔毛羽扇豆在两种土壤之间的净二氧化碳吸收量没有变化。钙和铁的分配要么是胁迫响应的结果，要么其本身就是一种胁迫响应。与不耐受品种相比，柔毛羽扇豆和一些埃及基因型的可溶性钙浓度有所不同，尽管埃及基因型吉萨1（Giza 1）的钙分配没有明显差异。在施用石灰的土壤中，吉萨1茎中的Fe3+浓度保持在与在未施用石灰的土壤中生长的植株相当的水平上，而一个不耐受基因型的茎[Fe3+]则增加了。在这些条件下，吉萨1叶组织中植物铁中Fe2+的百分比增加；不耐受基因型的则减少。本文讨论了埃及基因型通过这些机制产生的潜在耐受性以及基于营养的筛选可能性。
