Pastor Jesús, Hernández Ana Jesus, Prieto Nuria, Fernández-Pascual Mercedes
Centro de Ciencias Medioambientales, CSIC. C/Serrano, 115 dup. 28006-Madrid, Spain.
J Plant Physiol. 2003 Dec;160(12):1457-65. doi: 10.1078/0176-1617-01007.
Lupinus albus L. is a leguminous plant that is starting to generate interest for the phytoremediation soils showing intermediate metal pollution. Among these metals, Zn causes major phytotoxicity problems and is common in polluted soils of central Spain. The purpose of this study was to explore the nutritional behaviour of this plant species towards increasing Zn concentrations in two calcic luvisol soils: a normal basic soil and a decalcified acid soil. For this purpose the effects of different Zn concentrations on mineral nutrition, growth, nodulation and nitrogenase activity of nodulated Lupinus albus cv. Multolupa plants has been investigated. A 12-week trial was performed in pots under greenhouse conditions. In each soil, four replicate pots were set up per treatment (100, 150, 300, 500 and 700 ppm Zn). Seeds were inoculated with a Bradyrhizobium sp. (Lupinus) strain ISLU-16. Statistical analysis of data revealed significant effects of soil Zn on grown, plant mineral composition and nodulation. Lupin growth was better in acid soil than in basic soil with the low dose of Zn applied, although plant growth in acid soil was severely affected from 300 ppm Zn, where the pH of the soil was 4.7. Zn application produce nutritional imbalances, especially with the higher dose added. Most of Zn accumulation occurred in the roots in both types of soils. In acid soil, lupin absorbs high amounts of Zn in both root (4650 ppm) and aerial part (3605 ppm), when the doses of Zn applied was 300 ppm. This feature permits Lupinus albus cv. Multolupa to be considered as potential phytoremediator and also for the revegetation of degraded landfill areas with slightly acid or neutral soils polluted with Zn.
白羽扇豆是一种豆科植物，对于中度金属污染土壤的植物修复正开始引起人们的关注。在这些金属中，锌会引发主要的植物毒性问题，并且在西班牙中部的污染土壤中很常见。本研究的目的是探究该植物物种在两种石灰性淋溶土（一种正常的碱性土壤和一种脱钙酸性土壤）中，面对不断增加的锌浓度时的营养行为。为此，研究了不同锌浓度对结瘤白羽扇豆品种Multolupa植株的矿质营养、生长、结瘤和固氮酶活性的影响。在温室条件下于花盆中进行了为期12周的试验。在每种土壤中，每个处理设置四个重复花盆（锌浓度为100、150、300、500和700 ppm）。种子用慢生根瘤菌属（羽扇豆）菌株ISLU - 16进行接种。数据的统计分析表明，土壤锌对生长、植物矿质组成和结瘤有显著影响。在低锌用量时，羽扇豆在酸性土壤中的生长比在碱性土壤中更好，不过当土壤锌浓度达到300 ppm（此时土壤pH为4.7）时，酸性土壤中的植物生长受到严重影响。施用锌会导致营养失衡，尤其是添加较高剂量时。在两种土壤类型中，大部分锌积累都发生在根部。在酸性土壤中，当施用的锌剂量为300 ppm时，羽扇豆在根部（4650 ppm）和地上部分（3605 ppm）都吸收了大量的锌。这一特性使得Multolupa品种的白羽扇豆被视为潜在的植物修复剂，也可用于对受锌污染的微酸性或中性退化垃圾填埋场进行植被恢复。
