Holloway M David, Lantin Maria
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Ann Bot. 2002 Apr;89(4):409-17. doi: 10.1093/aob/mcf061.
A kinetic model is developed for cell differentiation in the fern gametophyte to test hypotheses on the role of spatially patterned plasmodesmata networks in development. Of particular interest is the establishment and maintenance of apical cell type in a single cell, with concurrent suppression of this character in all other cells (apical dominance). Steps towards understanding apical cell localization in geometrically simple gametophytes may shed light on the establishment and maintenance of apical meristems in higher plants. The model, based on the plasmodesmata maps of Tilney and colleagues and involving kinetics for a requisite minimum of two morphogens. successfully produces the apical/non-apical cell differentiation patterns of normal development, and redifferentiation due to cell isolation, in six stages from 0-30 d of development. Our results indicate that increasing apical cell plasmodesmata number, as development progresses, is not required for effective transport across apical cell walls in maintaining apical dominance.
为了验证关于空间模式化的胞间连丝网络在蕨类配子体发育中作用的假设，建立了一个关于蕨类配子体细胞分化的动力学模型。特别令人感兴趣的是单个细胞中顶端细胞类型的建立和维持，同时在所有其他细胞中抑制这种特征（顶端优势）。了解几何结构简单的配子体中顶端细胞定位的研究进展可能有助于揭示高等植物顶端分生组织的建立和维持机制。该模型基于蒂尔尼及其同事的胞间连丝图谱，涉及至少两种形态发生素的动力学，成功地在发育的0至30天的六个阶段产生了正常发育的顶端/非顶端细胞分化模式，以及由于细胞分离导致的再分化。我们的结果表明，随着发育的进行，增加顶端细胞胞间连丝数量对于维持顶端优势时跨顶端细胞壁的有效运输并非必需。
