Ahn S-J, Kho H-S, Lee S-W, Nahm D-S
Department of Orthodontics, College of Dentistry, Seoul National University, 28-22 Yunkeun-Dong, Chongro-Ku, Seoul 110-744, Korea, ROK.
J Dent Res. 2002 Jun;81(6):411-5. doi: 10.1177/154405910208100611.
Knowledge of salivary pellicles on orthodontic brackets provides a better understanding of microbial adherence. The aim of this study was to analyze the effects of bracket pellicles on the adherence of Streptococcus gordonii and Streptococcus mutans. Bracket pellicles were formed by the incubation of 4 kinds of orthodontic brackets with unstimulated whole saliva for 2 hrs, and analyzed by electrophoresis, immunodetection, and amino acid analysis. Binding assays were then performed by the incubation of tritium-labeled streptococci with the pellicle-transfer blots and orthodontic brackets. The results showed that low-molecular-weight mucin, alpha-amylase, secretory IgA, acidic proline-rich proteins, and cystatins adhered to all kinds of brackets, though the amino acid composition of pellicles differed between bracket types. Some of these proteins increased the binding of S. gordonii to saliva-coated brackets. However, salivary pellicles decreased the binding of S. mutans. Collectively, salivary pellicles were found to play a significant role in the initial adhesion of oral streptococci to orthodontic brackets.
了解正畸托槽上的唾液薄膜有助于更好地理解微生物的黏附。本研究的目的是分析托槽薄膜对戈登链球菌和变形链球菌黏附的影响。通过将4种正畸托槽与未刺激的全唾液孵育2小时形成托槽薄膜，并通过电泳、免疫检测和氨基酸分析进行分析。然后通过将氚标记的链球菌与薄膜转移印迹和正畸托槽孵育来进行结合试验。结果表明，低分子量粘蛋白、α-淀粉酶、分泌型IgA、富含酸性脯氨酸的蛋白质和胱抑素附着于所有类型的托槽，尽管不同类型托槽的薄膜氨基酸组成有所不同。其中一些蛋白质增加了戈登链球菌与唾液包被托槽的结合。然而，唾液薄膜减少了变形链球菌的结合。总的来说，发现唾液薄膜在口腔链球菌对正畸托槽的初始黏附中起重要作用。
