Lima Emilena Maria Castor Xisto, Koo Hyun, Vacca Smith Anne M, Rosalen Pedro Luiz, Del Bel Cury Altair Antoninha
Department of Prosthodontics and Periodontology, Faculty of Dentistry of Piracicaba, University of Campinas, Piracicaba, SP, Brazil.
Clin Oral Implants Res. 2008 Aug;19(8):780-5. doi: 10.1111/j.1600-0501.2008.01524.x.
The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO(2)) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO(2) were compared to those formed on hydroxyapatite (HA) surface.
The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS-PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P<0.05. In general, the electrophoretic analysis of the pellicles formed on HA, Ti and ZrO(2) revealed few qualitative differences of the composition of proteins of the pellicles formed on HA, Ti and ZrO(2) surfaces. Pellicle components identified included amylase, IgA, IgG, albumin, fibronectin and fibrinogen. The number of S. mutans cells adhered to uncoated Ti and ZrO(2) was significantly higher than those adhered to HA (P<0.05). In contrast, lower number of A. naeslundii cells adhered to uncoated Ti and ZrO(2) than to HA (P<0.05). However, the presence of saliva and saliva+serum pellicles greatly reduced the number of S. mutans cells bound to each of the surfaces. The data showed that Ti and ZrO(2) display similar pellicle protein composition and bacterial binding properties; however, significant differences were observed when both materials were compared to HA.
本研究旨在确定在钛（Ti）和氧化锆陶瓷（ZrO₂）表面形成的薄膜中唾液和血清蛋白的模式，以及细菌细胞粘附于实验性薄膜的能力。此外，将Ti和ZrO₂上薄膜的蛋白质谱和细菌结合特性与在羟基磷灰石（HA）表面形成的薄膜进行比较。
通过将材料与全唾液、血清或唾液+血清孵育在体外形成薄膜。通过SDS-PAGE和免疫检测研究每个薄膜中的蛋白质组成。通过闪烁计数法测定放射性标记的变形链球菌和内氏放线菌对未涂层表面和实验性薄膜的粘附。使用方差分析和Tukey检验进行统计分析，显著性水平为P<0.05。总体而言，对在HA、Ti和ZrO₂上形成的薄膜进行的电泳分析显示，在HA、Ti和ZrO₂表面形成的薄膜蛋白质组成在定性上几乎没有差异。鉴定出的薄膜成分包括淀粉酶、IgA、IgG、白蛋白、纤连蛋白和纤维蛋白原。粘附在未涂层Ti和ZrO₂上的变形链球菌细胞数量显著高于粘附在HA上的数量（P<0.05）。相比之下，粘附在未涂层Ti和ZrO₂上的内氏放线菌细胞数量低于粘附在HA上的数量（P<0.05）。然而，唾液和唾液+血清薄膜的存在大大减少了结合在每个表面上的变形链球菌细胞数量。数据表明，Ti和ZrO₂显示出相似的薄膜蛋白组成和细菌结合特性；然而，当将这两种材料与HA进行比较时，观察到显著差异。
