Palosaari H, Ding Y, Larmas M, Sorsa T, Bartlett J D, Salo T, Tjäderhane L
Institute of Dentistry, PO Box 5281, 90014 University of Oulu, Oulu, Finland.
J Dent Res. 2002 May;81(5):354-9. doi: 10.1177/154405910208100513.
MT1-MMP is a cell-membrane-bound metalloenzyme that activates other proMMPs such as proMMP-2 and -13. We studied MT1-MMP expression in mature human odontoblasts and pulp tissue, the regulation of MT1-MMP expression by growth factors TGF-beta1 and BMP-2, and the activation of odontoblast-derived MMP-20 by MT1-MMP. MT1-MMP mRNA is expressed by native and cultured mature human odontoblasts and pulp tissue. Western blot analysis of human odontoblasts and pulp tissue detects 65- and 51-kDa pro- and active forms of MT1-MMP, and smaller truncated MT1-MMP forms. BMP-2 down-regulates MT1-MMP expression in odontoblasts and pulp tissue, while TGF-beta1, alone or with BMP-2, decreases MT1-MMP mRNA levels only slightly. We also demonstrate that MT1-MMP is capable of converting proMMP-20 into a form corresponding to the active MMP-20. In conclusion, this study demonstrates the expression and differential regulation of MT1-MMP in human dentin-pulp complex cells, and the activation of MMP-20 by MT1-MMP.
基质金属蛋白酶-1（MT1-MMP）是一种细胞膜结合金属酶，可激活其他前基质金属蛋白酶，如前基质金属蛋白酶-2和-13。我们研究了MT1-MMP在成熟人成牙本质细胞和牙髓组织中的表达、生长因子转化生长因子-β1（TGF-β1）和骨形态发生蛋白-2（BMP-2）对MT1-MMP表达的调节，以及MT1-MMP对成牙本质细胞源性基质金属蛋白酶-20（MMP-20）的激活作用。MT1-MMP信使核糖核酸（mRNA）在天然和培养的成熟人成牙本质细胞及牙髓组织中表达。对人成牙本质细胞和牙髓组织进行蛋白质免疫印迹分析，可检测到65千道尔顿和51千道尔顿的MT1-MMP前体和活性形式，以及较小的截短型MT1-MMP形式。BMP-2下调成牙本质细胞和牙髓组织中MT1-MMP的表达，而TGF-β1单独或与BMP-2一起仅轻微降低MT1-MMP mRNA水平。我们还证明MT1-MMP能够将前MMP-20转化为与活性MMP-20相对应的形式。总之，本研究证明了MT1-MMP在人牙本质-牙髓复合体细胞中的表达及差异调节，以及MT1-MMP对MMP-20的激活作用。
