Zou Jian, Shan Song-Hua, Yao Long-Tao, Gong Zu-Xun
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Jul;34(4):439-44.
The complete F gene of SF02 of goose paramyxovirus (GPV) has been cloned and analyzed. The sequence analysis demonstrated that the F gene of SF02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of F gene has the same deduced amino acid sequence, (112)R-R-Q-K-R-F(117), as the velogenic (highly pathogenic) strain of newcastle disease virus. The latter correlated with the virulence of the isolate in biological assays. The F gene of SF02 isolate with the domestic standard velogenic strain NDV, F48E9, shared 86.5% homology in nucleotide and 90.8% homology in amino acid sequences. The SF02 isolate is closer to some NDV strains prevalent in Taiwan and West-European countries in recent years. Based on the F gene sequence a multiplex RT-PCR method has been developed. It could be used for the discrimination of GPV from NDV.
已对鹅副粘病毒（GPV）SF02的完整F基因进行了克隆和分析。序列分析表明，SF02的F基因包含1662个核苷酸，编码553个氨基酸，其F基因的裂解激活位点具有与新城疫病毒强毒株相同的推导氨基酸序列，即(112)R-R-Q-K-R-F(117)。后者与生物学试验中分离株的毒力相关。SF02分离株的F基因与国内标准强毒株新城疫病毒F48E9在核苷酸上具有86.5%的同源性，在氨基酸序列上具有90.8%的同源性。SF02分离株与近年来在台湾和西欧国家流行的一些新城疫病毒株关系更近。基于F基因序列开发了一种多重RT-PCR方法。它可用于区分鹅副粘病毒和新城疫病毒。
