Yang Tao, Gao Yan-Kun, Chen Jiang-Ye
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Jul;34(4):520-5.
hPFTAIRE1 is a member of Cdc2-related kinase family localized in cytoplasm. To search its substrates and regulatory proteins, the hPFTAIRE1 was fused to LexA and used as bait to screen a human brain LexA two-hybrid library. In this screening, 7 hPFTAIRE1 interacting proteins, including KIAA0202, were obtained. The interaction between the KIAA0202 and the hPFTAIRE1 was confirmed by using immunoprecipitation. The KIAA0202 is a 508 aa protein and is a member of septin family. The central portion of the KIAA0202 is a GTP-CDC domain, which is conserved among almost all septins. Its C-terminus is a coiled-coil structure and its N-terminus is a variable region. Our results suggest that the KIAA0202 may play important roles in functional regulation of hPFTAIRE1.
hPFTAIRE1是一种定位于细胞质的Cdc2相关激酶家族成员。为了寻找其底物和调节蛋白，将hPFTAIRE1与LexA融合，并用作诱饵来筛选人脑LexA双杂交文库。在这次筛选中，获得了7种与hPFTAIRE1相互作用的蛋白，包括KIAA0202。通过免疫沉淀证实了KIAA0202与hPFTAIRE1之间的相互作用。KIAA0202是一种含508个氨基酸的蛋白，是septin家族的成员。KIAA0202的中央部分是一个GTP-CDC结构域，几乎在所有septin中都保守。其C末端是一个卷曲螺旋结构，N末端是一个可变区域。我们的结果表明，KIAA0202可能在hPFTAIRE1的功能调节中发挥重要作用。
