人septin蛋白SEPT8（KIAA0202）新剪接异构体的分离、特性鉴定及表达分析

Isolation of new splice isoforms, characterization and expression analysis of the human septin SEPT8 (KIAA0202).

作者信息

Bläser Susanne, Jersch Katrin, Hainmann Ina, Zieger Wolfgang, Wunderle Daniela, Busse Anja, Zieger Barbara

机构信息

Department of Pediatrics and Adolescent Medicine, University of Freiburg, Children's Hospital, Mathildenstrasse 1, D-79106 Freiburg, Germany.

出版信息

Gene. 2003 Jul 17;312:313-20. doi: 10.1016/s0378-1119(03)00635-8.

DOI:10.1016/s0378-1119(03)00635-8

PMID:12909369

Abstract

SEPT8 (KIAA0202) is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Using the yeast two-hybrid system and the glutathione-S-transferase pull-down assay we previously had identified the SEPT8 (KIAA0202) as interaction partner of the human septin SEPT5 (cell division cycle related-1, CDCrel-1). Since the complete cDNA sequence of the human septin SEPT8 (KIAA0202) was not known at that time, we isolated new 5' and 3' cDNA sequence of SEPT8 (KIAA0202) by screening three different cDNA libraries. In addition, we performed the characterization of SEPT8 (KIAA0202) and identified new splice variants of SEPT8 (KIAA0202). The expression pattern of SEPT8 (KIAA0202) and its interaction partner SEPT5 (CDCrel-1) is illustrated.

摘要

SEPT8（KIAA0202）是高度保守的septin家族的成员。Septin是与膜相关的GTP酶，参与胞质分裂和细胞形态发生。我们之前利用酵母双杂交系统和谷胱甘肽-S-转移酶下拉分析，将SEPT8（KIAA0202）鉴定为人septin SEPT5（细胞分裂周期相关蛋白1，CDCrel-1）的相互作用伙伴。由于当时人类septin SEPT8（KIAA0202）的完整cDNA序列尚不清楚，我们通过筛选三个不同的cDNA文库，分离出了SEPT8（KIAA0202）新的5'和3' cDNA序列。此外，我们对SEPT8（KIAA0202）进行了表征，并鉴定出SEPT8（KIAA0202）的新剪接变体。文中展示了SEPT8（KIAA0202）及其相互作用伙伴SEPT5（CDCrel-1）的表达模式。