Bläser Susanne, Jersch Katrin, Hainmann Ina, Wunderle Daniela, Zgaga-Griesz Andrea, Busse Anja, Zieger Barbara
Children's Hospital, University of Freiburg, Mathildenstr. 1, Freiburg, Germany.
FEBS Lett. 2002 May 22;519(1-3):169-72. doi: 10.1016/s0014-5793(02)02749-7.
Septins are evolutionary conserved cytoskeletal GTPases forming heteropolymer complexes involved in cytokinesis and other cellular processes. CDCrel-1 (cell division cycle related-1) is a recently cloned and characterized human septin which is highly expressed in non-dividing cells, such as neurons. Using a yeast two-hybrid system we demonstrate that CDCrel-1 partners with another uncharacterized human septin, KIAA0202. The interaction of CDCrel-1 and KIAA0202 was confirmed in the human leukemia cell line K-562 using pull-down assays with a KIAA0202-glutathione S-transferase fusion protein and by immunoprecipitation of the CDCrel-1-KIAA0202 complex with an anti-KIAA0202 antibody. Expression studies of the two human septins revealed a concomitant expression of both proteins in certain cells.
Septin蛋白是进化保守的细胞骨架GTP酶，可形成参与胞质分裂和其他细胞过程的异源聚合物复合物。CDCrel-1（细胞分裂周期相关蛋白1）是最近克隆和鉴定的人类Septin蛋白，在非分裂细胞（如神经元）中高度表达。利用酵母双杂交系统，我们证明CDCrel-1与另一种未鉴定的人类Septin蛋白KIAA0202相互作用。通过使用KIAA0202-谷胱甘肽S-转移酶融合蛋白进行下拉实验，以及用抗KIAA0202抗体对CDCrel-1-KIAA0202复合物进行免疫沉淀，在人白血病细胞系K-562中证实了CDCrel-1和KIAA0202的相互作用。对这两种人类Septin蛋白的表达研究表明，这两种蛋白在某些细胞中同时表达。
