携带前S表位的重组乙型肝炎病毒表面抗原在毕赤酵母中的表达。

Expression of the Recombinant Hepatitis B Virus Surface Antigen Carrying PreS Epitopes in Pichia pastoris.

作者信息

Yang Jian-Ying, Hui Jing-Yi, Li Guang-Di, Wang Yuan, Yuan Han-Ying, Li Yu-Yang

机构信息

Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.

出版信息

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(2):139-144.

PMID:12098790

Abstract

Many studies have suggested that hepatitis B surface antigen(HBsAg) including PreS sequences could be an ideal candidate for highly effective hepatitis B virus vaccine. Modified surface antigens S1S, SS1 and S2S which carried PreS epitopes, were expressed in Pichia pastoris. The characterization of antigenicity and particle assembly demonstrated that the expression products could be assembled into particles which presented S, PreS1 or PreS2 antigenicity, respectively. The expression was more efficient than that in Saccharomyces cerevisiae.

摘要

许多研究表明，包含前S序列的乙肝表面抗原（HBsAg）可能是高效乙肝病毒疫苗的理想候选物。携带前S表位的修饰表面抗原S1S、SS1和S2S在毕赤酵母中表达。抗原性和颗粒组装特性表明，表达产物可分别组装成呈现S、前S1或前S2抗原性的颗粒。其表达效率高于在酿酒酵母中的表达效率。