Vellanki Ravi Nagaraj, Komaravelli Narayana, Tatineni Radhika, Mangamoori Lakshmi Narasu
Centre for Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India.
Biotechnol Lett. 2007 Feb;29(2):313-8. doi: 10.1007/s10529-006-9242-0. Epub 2006 Nov 29.
An expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant hepatitis B surface antigen was synthesized by cloning hepatitis B virus 'S' gene under the control of glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. Hepatitis B surface antigen was constitutively expressed, was stable and exhibited approximately 20-22 nm particle formation. Stability and absence of toxicity to the host with the expression vector indicates the expression system can be applied for large-scale production.
将由毕赤酵母基因构建的表达载体用于酿酒酵母中的异源基因表达。通过在酿酒酵母中，将乙型肝炎病毒“S”基因克隆到毕赤酵母甘油醛-3-磷酸脱氢酶（GAP）启动子控制下，合成重组乙型肝炎表面抗原。乙型肝炎表面抗原组成性表达，稳定且呈现出约20 - 22纳米的颗粒形成。该表达载体的稳定性以及对宿主无毒性表明该表达系统可用于大规模生产。
